Absorption of plasma proteins from peritoneal cavity of normal rats

Journal Article · Sat Apr 01 00:00:00 EST 1989 · Am. J. Physiol.; (United States)

OSTI ID:6101000

Regoeczi, E; Zaimi, O; Chindemi, P A; Charlwood, P A

The present study was undertaken to examine whether the uptake of plasma proteins from the peritoneal cavity is quantitative so that tracers could be introduced that way for measuring their turnover. To this end, the metabolic behavior of seven homologous plasma proteins, labeled with 125I, was compared in rats after intravenous or intraperitoneal administration. The animals were maintained under physiological conditions. Total body radiation measurements showed that the degradation rates of albumin, immunoglobulins A and G, alpha 1-macroglobulin, and transferrin were the same regardless of the route of injection. This implies that these proteins are quantitatively absorbed from the peritoneum without undergoing modifications. The half-life of intraperitoneally injected alpha 1-acid glycoprotein was consistently shorter by an average 9%, thus suggesting that this protein becomes slightly altered if introduced that way. Only one-half of intraperitoneally injected fibrinogen survived normally, whereas the other underwent rapid degradation. The surviving molecules had the same half-life as fibrinogen injected intravenously. The fraction of surviving fibrinogen could be augmented by mixing the dose with serum. Within a wide range of concentrations and quantities injected, the degradation rate of transferrin remained the same. Analysis by deconvolution of the plasma curves of albumin and alpha 1-macroglobulin absorbed from the peritoneum showed that the transport process was independent of protein size and, at least up to 35 mg, of the amount injected. According to the same technique, intraperitoneally administered diferric transferrin retained its iron during passage into the circulation.

• Research Organization: McMaster Univ. Health Sciences Centre, Hamilton, Ontario (Canada)
• OSTI ID: 6101000
• Journal Information: Am. J. Physiol.; (United States), Vol. 256
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ALBUMINS
METABOLISM
FIBRINOGEN
GLOBULINS
GLYCOPROTEINS
IMMUNOGLOBULINS
TRANSFERRIN
ABSORPTION
BIOLOGICAL HALF-LIFE
BLOOD
INTRAPERITONEAL INJECTION
INTRAVENOUS INJECTION
IODINE
MEMBRANE TRANSPORT
PERITONEUM
RATS
TRACER TECHNIQUES
ANIMALS
BIOLOGICAL MATERIALS
BLOOD COAGULATION FACTORS
BODY FLUIDS
COAGULANTS
DRUGS
ELEMENTS
GLOBULINS-BETA
HALOGENS
HEMATOLOGIC AGENTS
HEMOSTATICS
INJECTION
INTAKE
ISOTOPE APPLICATIONS
MAMMALS
MATERIALS
MEMBRANES
METALLOPROTEINS
NONMETALS
ORGANIC COMPOUNDS
PROTEINS
RODENTS
SEROUS MEMBRANES
VERTEBRATES
550501* - Metabolism- Tracer Techniques