Thiamine metabolism in folate deficient rats
Abstract
Folate status (FS) and resultant alterations in thiamine status (TS) were evaluated in weanling rats fed either 17% amino acids (RHAA); 14% amino acids (LOGLU); 20% Vitamin Free casein (VFC) + 8% gelatin (HICG); 10% VFC + 4% gelatin + 0.3% methionine (CGM); or 10% VFC + 4 % gelatin (LOCG). Diets were fed with and without 8 mg FA/kg diet. HICG diet contained 54 ug/kg endogenous folate, the CGM and LOCG, 27 ug/kg, RHAA and LOGLU were folate free. FS was assessed by growth rate, hematology, formiminoglutamic acid excretion following a histidine load and tissue folate levels. TS was assessed by determining the fate of oral /sup 3/H-labeled and intravenous /sup 14/C-labeled thiamine over a six hour test period and by measurement of blood transketolase activity (TKA) and TPP effect (TPPE). TKA and TPPE were measured by an enzymatic single-point assay developed during these investigations.
- Authors:
- Publication Date:
- Research Org.:
- California Univ., Davis (USA)
- OSTI Identifier:
- 6100777
- Resource Type:
- Thesis/Dissertation
- Resource Relation:
- Other Information: Thesis (Ph. D.)
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; FOLIC ACID; NUTRITIONAL DEFICIENCY; THIAMINE; METABOLISM; TRANSFERASES; ENZYME ACTIVITY; ANIMAL GROWTH; CARBON 14 COMPOUNDS; DIET; METHIONINE; RADIOENZYMATIC ASSAY; RATS; TRACER TECHNIQUES; TRITIUM COMPOUNDS; AMINES; AMINO ACIDS; ANIMALS; AROMATICS; AZAARENES; AZINES; AZOLES; CARBOXYLIC ACIDS; DRUGS; ENZYMES; GROWTH; HEMATINICS; HEMATOLOGIC AGENTS; HETEROCYCLIC COMPOUNDS; HYDROXY COMPOUNDS; ISOTOPE APPLICATIONS; LABELLED COMPOUNDS; LIPOTROPIC FACTORS; MAMMALS; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; ORGANIC SULFUR COMPOUNDS; PTERIDINES; PYRIMIDINES; RODENTS; THIAZOLES; VERTEBRATES; VITAMIN B GROUP; VITAMINS; 550501* - Metabolism- Tracer Techniques
Citation Formats
Walzem, R L. Thiamine metabolism in folate deficient rats. United States: N. p., 1987.
Web.
Walzem, R L. Thiamine metabolism in folate deficient rats. United States.
Walzem, R L. 1987.
"Thiamine metabolism in folate deficient rats". United States.
@article{osti_6100777,
title = {Thiamine metabolism in folate deficient rats},
author = {Walzem, R L},
abstractNote = {Folate status (FS) and resultant alterations in thiamine status (TS) were evaluated in weanling rats fed either 17% amino acids (RHAA); 14% amino acids (LOGLU); 20% Vitamin Free casein (VFC) + 8% gelatin (HICG); 10% VFC + 4% gelatin + 0.3% methionine (CGM); or 10% VFC + 4 % gelatin (LOCG). Diets were fed with and without 8 mg FA/kg diet. HICG diet contained 54 ug/kg endogenous folate, the CGM and LOCG, 27 ug/kg, RHAA and LOGLU were folate free. FS was assessed by growth rate, hematology, formiminoglutamic acid excretion following a histidine load and tissue folate levels. TS was assessed by determining the fate of oral /sup 3/H-labeled and intravenous /sup 14/C-labeled thiamine over a six hour test period and by measurement of blood transketolase activity (TKA) and TPP effect (TPPE). TKA and TPPE were measured by an enzymatic single-point assay developed during these investigations.},
doi = {},
url = {https://www.osti.gov/biblio/6100777},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Thu Jan 01 00:00:00 EST 1987},
month = {Thu Jan 01 00:00:00 EST 1987}
}