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Title: Metabolism of inositol(1,4,5)trisphosphate by a soluble enzyme fraction from pea (Pisum sativum) roots

Abstract

Metabolism of the putative messenger molecule D-myo-inositol(1,4,5)trisphosphate (Ins(1,4,5)P{sub 3}) in plant cells has been studied using a soluble fraction from pea (pisum sativum) roots as enzyme source and (5-{sup 32}P)Ins(1,4,5)P{sub 3} and (2-{sup 3}H)Ins(1,4,5)P{sub 3} as tracers. Ins(1,4,5)P{sub 3} was rapidly converted into both lower and higher inositol phosphates. The major dephosphorylation product was inositol (4,5) bisphosphate (Ins(4,5)P{sub 2}) whereas inositol(1,4)bisphosphate (Ins(1,4)P{sub 2}) was only present in very small quantities throughout a 15 minute incubation period. In addition to these compounds, small amounts of nine other metabolites were produced including inositol and inositol(1,4,5,X)P{sub 4}. Dephosphorylation of Ins(1,4,5)P{sub 3} to Ins(4,5)P{sub 2} was dependent on Ins(1,4,5)P{sub 3} concentration and was partially inhibited by the phosphohydrolase inhibitors 2,3-diphosphoglycerate, glucose 6-phosphate, and p-nitrophenylphosphate. Conversion of Ins(1,4,5)P{sub 3} to Ins(4,5)P{sub 2} and Ins(1,4,5,X)P{sub 4} was inhibited by 55 micromolar Ca{sup 2+}. This study demonstrates that enzymes are present in plant tissues which are capable of rapidly converting Ins(1,4,5)P{sub 3} and that pathways of inositol phosphate metabolism exist which may prove to be unique to the plant kingdom.

Authors:
; ;  [1];  [2];  [3]
  1. John Innes Inst., Norwich (England)
  2. John Innes Inst., Norwich (England) Univ. of East Anglia, Norwich (England)
  3. Univ. of East Anglia, Norwich (England)
Publication Date:
OSTI Identifier:
6098661
Resource Type:
Journal Article
Journal Name:
Plant Physiology; (United States)
Additional Journal Information:
Journal Volume: 95:2; Journal ID: ISSN 0032-0889
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; PISUM; BIOLOGICAL PATHWAYS; CALCIUM COMPOUNDS; CATIONS; ENZYME ACTIVITY; INOSITOLS; METABOLISM; METABOLITES; PHOSPHORUS 32; PLANT CELLS; ROOTS; TRACER TECHNIQUES; TRITIUM COMPOUNDS; ALKALINE EARTH METAL COMPOUNDS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; CARBOHYDRATES; CHARGED PARTICLES; DAYS LIVING RADIOISOTOPES; HYDROGEN COMPOUNDS; IONS; ISOTOPE APPLICATIONS; ISOTOPES; LEGUMINOSAE; LIGHT NUCLEI; MAGNOLIOPHYTA; MAGNOLIOPSIDA; MONOSACCHARIDES; NUCLEI; ODD-ODD NUCLEI; ORGANIC COMPOUNDS; PHOSPHORUS ISOTOPES; PLANTS; RADIOISOTOPES; SACCHARIDES; 551001* - Physiological Systems- Tracer Techniques

Citation Formats

Drobak, B K, Watkins, P A.C., Roberts, K, Chattaway, J A, and Dawson, A P. Metabolism of inositol(1,4,5)trisphosphate by a soluble enzyme fraction from pea (Pisum sativum) roots. United States: N. p., 1991. Web. doi:10.1104/pp.95.2.412.
Drobak, B K, Watkins, P A.C., Roberts, K, Chattaway, J A, & Dawson, A P. Metabolism of inositol(1,4,5)trisphosphate by a soluble enzyme fraction from pea (Pisum sativum) roots. United States. https://doi.org/10.1104/pp.95.2.412
Drobak, B K, Watkins, P A.C., Roberts, K, Chattaway, J A, and Dawson, A P. 1991. "Metabolism of inositol(1,4,5)trisphosphate by a soluble enzyme fraction from pea (Pisum sativum) roots". United States. https://doi.org/10.1104/pp.95.2.412.
@article{osti_6098661,
title = {Metabolism of inositol(1,4,5)trisphosphate by a soluble enzyme fraction from pea (Pisum sativum) roots},
author = {Drobak, B K and Watkins, P A.C. and Roberts, K and Chattaway, J A and Dawson, A P},
abstractNote = {Metabolism of the putative messenger molecule D-myo-inositol(1,4,5)trisphosphate (Ins(1,4,5)P{sub 3}) in plant cells has been studied using a soluble fraction from pea (pisum sativum) roots as enzyme source and (5-{sup 32}P)Ins(1,4,5)P{sub 3} and (2-{sup 3}H)Ins(1,4,5)P{sub 3} as tracers. Ins(1,4,5)P{sub 3} was rapidly converted into both lower and higher inositol phosphates. The major dephosphorylation product was inositol (4,5) bisphosphate (Ins(4,5)P{sub 2}) whereas inositol(1,4)bisphosphate (Ins(1,4)P{sub 2}) was only present in very small quantities throughout a 15 minute incubation period. In addition to these compounds, small amounts of nine other metabolites were produced including inositol and inositol(1,4,5,X)P{sub 4}. Dephosphorylation of Ins(1,4,5)P{sub 3} to Ins(4,5)P{sub 2} was dependent on Ins(1,4,5)P{sub 3} concentration and was partially inhibited by the phosphohydrolase inhibitors 2,3-diphosphoglycerate, glucose 6-phosphate, and p-nitrophenylphosphate. Conversion of Ins(1,4,5)P{sub 3} to Ins(4,5)P{sub 2} and Ins(1,4,5,X)P{sub 4} was inhibited by 55 micromolar Ca{sup 2+}. This study demonstrates that enzymes are present in plant tissues which are capable of rapidly converting Ins(1,4,5)P{sub 3} and that pathways of inositol phosphate metabolism exist which may prove to be unique to the plant kingdom.},
doi = {10.1104/pp.95.2.412},
url = {https://www.osti.gov/biblio/6098661}, journal = {Plant Physiology; (United States)},
issn = {0032-0889},
number = ,
volume = 95:2,
place = {United States},
year = {Fri Feb 01 00:00:00 EST 1991},
month = {Fri Feb 01 00:00:00 EST 1991}
}