Molecular mechanism of extinction of liver-specific functions in mouse hepatoma x rat fibroblast hybrids: extinction of the albumin gene
- Oak Ridge National Lab., TN
Hybrids formed by the fusion of mouse hepatoma (BWTG3) and rat fibroblast (JF1) cells exhibit the extinction of mouse albumin and ..cap alpha..-fetoprotein synthesis. Karyotype analyses suggest that all parental chromosomes are present in the hybrids. The extinction, therefore, of mouse hepatocyte genes is attributed to the inhibitory action of the rat genome. In these studies, we show that these hybrids possess and express the mouse ..beta..-glucyronidase gene (which is encoded on the same chromosome as the mouse albumin and ..cap alpha..-fetoprotein gene), and we present data of Southern blot analysis which demonstrate that such hybrids have indeed retained both mouse and rat albumin DNA sequences. In addition, using mouse albumin cDNA, we have shown by cDNA-RNA reassociation kinetics that albumin mRNA is virtually absent in these hybrids. We conclude from these studies that the extinction of albumin synthesis involves a mechanism which results in the loss of cytoplasmic albumin mRNA.
- OSTI ID:
- 6097932
- Journal Information:
- Somatic Cell Genet.; (United States), Vol. 8:3
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
FIBROBLASTS
HYBRIDIZATION
TUMOR CELLS
ALBUMINS
BIOSYNTHESIS
DNA
ENZYME ACTIVITY
EXPERIMENTAL DATA
GENES
GLUCURONIDASE
HEPATOMAS
KARYOTYPE
MESSENGER-RNA
MICE
PROTEINS
RATS
RNA
TEMPERATURE DEPENDENCE
ANIMAL CELLS
ANIMALS
CONNECTIVE TISSUE CELLS
DATA
DISEASES
ENZYMES
GLYCOSYL HYDROLASES
HYDROLASES
INFORMATION
MAMMALS
NEOPLASMS
NUCLEIC ACIDS
NUMERICAL DATA
O-GLYCOSYL HYDROLASES
ORGANIC COMPOUNDS
RODENTS
SOMATIC CELLS
SYNTHESIS
VERTEBRATES
550400* - Genetics
550200 - Biochemistry