Metachromatic staining patterns of basic proline-rich proteins from rat and human saliva in sodium dodecyl sulfate-polyacrylamide gels

Journal Article · Mon Oct 01 00:00:00 EDT 1984 · J. Appl. Biochem.; (United States)

OSTI ID:6085511

Humphreys-Beher, M G; Wells, D J

A series of basic proteins, rich in proline, were isolated from the salivary secretions of humans and rats. These proteins underwent metachromasia after staining with Coomassie brilliant blue R-250 in sodium dodecyl sulfate-polyacrylamide gels. The technique of destaining gels in several changes of 10% acetic acid after a 30-min staining period is a rapid method of general utility for the identification of proline-rich proteins from total cell lysates from other sources besides saliva.

• Research Organization: Univ. of Alabama, Birmingham
• OSTI ID: 6085511
• Journal Information: J. Appl. Biochem.; (United States), Vol. 5-6
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
SALIVA
PROTEINS
TRITIUM COMPOUNDS
TRACER TECHNIQUES
DYES
ELECTROPHORESIS
MAN
PROLINE
RATS
SPECTROPHOTOMETRY
AMINES
AMINO ACIDS
ANIMALS
AZOLES
BIOLOGICAL MATERIALS
BODY FLUIDS
CARBOXYLIC ACIDS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
MATERIALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PRIMATES
PYRROLES
PYRROLIDINES
RODENTS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques