Effects of a calcium channel agonist on the electrical, ionic and secretory events in mouse pancreatic B-cells
The changes in pancreatic B-cell function produced by a Ca channel agonist, the dihydropyridine derivative CGP 28392, have been studied with mouse islets. CGP 28392 (5 microM) modified the electrical activity induced in B-cells by 10 mM glucose: the duration and the amplitude of the slow waves of membrane potential increased, but the overall spike activity decreased. Simultaneously, CGP 28392 markedly increased insulin release and UVCaS efflux, and slightly accelerated YWRb efflux from islet cells. These latter effects were abolished by omission of extracellular CaS . Qualitatively similar changes were observed at 15 mM glucose, whereas CGP 28392 was ineffective at 3 mM glucose. These results strongly suggest that an influx of CaS contributes to the slow waves of membrane potential triggered by glucose, and underline the importance of this influx of CaS for the control of insulin release by the sugar.
- Research Organization:
- Univ. of Saarland, Germany, F.R.
- OSTI ID:
- 6085407
- Journal Information:
- Biochem. Biophys. Res. Commun.; (United States), Vol. 2
- Country of Publication:
- United States
- Language:
- English
Similar Records
Inotropic effect, binding properties, and calcium flux effects of the calcium channel agonist CGP 28392 in intact cultured embryonic chick ventricular cells
Decreased insulin secretory response of pancreatic islets during culture in the presence of low glucose is associated with diminished /sup 45/Ca/sup 2 +/ net uptake, NADPH/NADP/sup +/ and GSH/GSSG ratios
Related Subjects
ANIMAL CELLS
BIOELECTRICITY
CALCIUM 45
MEMBRANE TRANSPORT
PYRIDINES
BIOLOGICAL EFFECTS
RUBIDIUM 86
CALCIUM COMPOUNDS
CATIONS
GLUCOSE
INSULIN
MICE
PANCREAS
RADIOISOTOPES
RUBIDIUM COMPOUNDS
ALDEHYDES
ALKALI METAL COMPOUNDS
ALKALI METAL ISOTOPES
ALKALINE EARTH ISOTOPES
ALKALINE EARTH METAL COMPOUNDS
ANIMALS
AZINES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CALCIUM ISOTOPES
CARBOHYDRATES
CHARGED PARTICLES
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
ELECTRICITY
ENDOCRINE GLANDS
EVEN-ODD NUCLEI
GLANDS
HETEROCYCLIC COMPOUNDS
HEXOSES
HORMONES
INTERMEDIATE MASS NUCLEI
IONS
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
MAMMALS
MINUTES LIVING RADIOISOTOPES
MONOSACCHARIDES
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PEPTIDE HORMONES
RODENTS
RUBIDIUM ISOTOPES
SACCHARIDES
VERTEBRATES
550301* - Cytology- Tracer Techniques