Estimation of transfused red cell survival using an enzyme-linked antiglobulin test

Journal Article · Sun Sep 01 00:00:00 EDT 1985 · Transfusion (Philadelphia); (United States)

DOI:https://doi.org/10.1046/j.1537-2995.1985.25586020109.x· OSTI ID:6085402

Kickler, T S; Smith, B; Bell, W; Drew, H; Baldwin, M; Ness, P M

An enzyme-linked antiglobulin test (ELAT) method was developed to estimate survival of transfused red cells. This procedure is based on a principle analogous to that of the Ashby technique were antigenically distinct red cells are transfused and their survival studied. The authors compared the ELAT survival to the V Chromium method (V Cr) in four patients. Three patients with hypoproliferative anemias showed T 1/2 by ELAT of 17.5, 18, and 17 days versus 18.5, 20, and 19 days by the V Cr method. A fourth patient with traumatic cardiac hemolysis had two studies performed. In this case, the ELAT showed a T 1/2 of 10 and 8.1 days while V Cr T 1/2 values were 11 and 10.5 days. The ELAT method for measuring red cell survival yielded data which agreed closely with the results of the V Cr method. Although V Cr is the accepted method for red cell survival, the ELAT method can be used to estimate transfused red cell survival.

• Research Organization: Johns Hopkins School of Medicine, Baltimore, MD
• OSTI ID: 6085402
• Journal Information: Transfusion (Philadelphia); (United States), Vol. 5
• Country of Publication: United States
• Language: English

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