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Title: Growth kinetics and yield study on Chlorella pyrenoidosa in chemically defined media

A Chlorella culture free from heterotrophic bacteria was obtained by eliminating the bacteria with successive use of antibiotics and agar plants. The purified Chlorella was cultured in chemically defined media. Under a photon flux (16.7 mw/cmS) similar to insolation, both heterotrophic and mixotrophic cultures were luxurious but the growth rates of autotrophic cultures were reduced substantially. The Chlorella culture grew most rapidly at 30 C in the absence of heterotrophic bacteria, and the highest specific growth rates were 1.43 x 10 h and 0.46 x 10 h for mixotrophic and autotrophic cultures, respectively. The highest photosynthetic efficiency over its growth period was 2.9% for autotrophic cultures. Elimination of heterotrophic bacteria from Chlorella cultures improved the algal growth rate as well as biomass yield significantly. A parasite of 0.1- m size was identified. The motile microorganism played an important role in the growth of the Chlorella and appeared to be common to green algae. 16 references, 2 tables.
Authors:
;
Publication Date:
OSTI Identifier:
6082552
Report Number(s):
CONF-8208164-
Journal ID: CODEN: DIMCA
Resource Type:
Conference
Resource Relation:
Journal Name: Dev. Ind. Microbiol.; (United States); Journal Volume: 24; Conference: 39. general meeting of the Society for Industrial Microbiology, St. Paul, MN, USA, 14 Aug 1982
Research Org:
Standard Oil Co., Naperville, IL
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 09 BIOMASS FUELS; CHLORELLA; GROWTH; PHOTOSYNTHESIS; RESOURCE POTENTIAL; EXPERIMENTAL DATA; PARASITES; ALGAE; CHEMICAL REACTIONS; DATA; INFORMATION; MICROORGANISMS; NUMERICAL DATA; PHOTOCHEMICAL REACTIONS; PLANTS; SYNTHESIS; UNICELLULAR ALGAE 550700* -- Microbiology; 140504 -- Solar Energy Conversion-- Biomass Production & Conversion-- (-1989)