Binding of Klebsiella K/sub 2/ LPS to specific antibody and cross-reactivity with pneumococcal group 19 polysaccharides (PS)

Conference · Fri May 01 00:00:00 EDT 1987 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:6079221

Li, J P; Ching, E D; Lee, C J

The authors have studied the chemical basis of the antigen-antibody reaction between Klebsiella K/sub 2/ LPS and specific antiserum, and cross-reactivity with pneumococcal group 19 PSs. K/sub 2/ LPS was isolated and purified from Klebsiella K/sub 2/ strain by the methods of hot phenol-water extraction, ultracentrifugation, treatment with enzymes and gel filtration. K/sub 2/ LPS was hydrolyzed with 1% acetic acid to produce 2 fractions, separated by Sephadex G-50 column. Fraction G-I was passed through Sepharose 4B column to separate into 3 peaks (4B-I, -II, and -III). Peak 4B-II showed a molecular size of Kd, 0.35 and 4B-III had Kd, 0.79. When K/sub 2/ LPS was injected to mice, IgM and IgG antibody responses were induced, as determined by ELISA. In contrast, with the 19F and 19A PSs, the K/sub 2/ LPS induced antibody was mostly IgM. They have studied the ability of PS fractions (4B-II, 4B-III and G-I) to inhibit the binding of K/sub 2/ LPS to specific IgM and IgG antibodies. Among these fractions, 4B-II PS showed a highest inhibition to the binding activity; approximately 1000-fold greater inhibiting ability than 4B-III and G-I PS. These results indicate that 4B-II PS may serve as an immunologic determinant of K/sub 2/ LPS. Pneumococcal 19F and 19A PSs induced low inhibition of the binding activity between K/sub 2/ LPS and specific IgM antibody. The fraction 4B-II as immunologic determinant of K/sub 2/ LPS was further characterized by /sup 125/I-K/sub 2/ LPS anti-serum and Western blotting electrophoresis.

• Research Organization: Federal Dept. of Agriculture, Bethesda, MD
• OSTI ID: 6079221
• Report Number(s): CONF-870644-; TRN: 87-037144
• Journal Information: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 46:6; Conference: 78. annual meeting of the American Society of Biological Chemists conference, Philadelphia, PA, USA, 7 Jun 1987
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
LIPOPOLYSACCHARIDES
ANTIGEN-ANTIBODY REACTIONS
BIOCHEMICAL REACTION KINETICS
POLYSACCHARIDES
ELECTROPHORESIS
FRACTIONATION
INHIBITION
IODINE 125
KLEBSIELLA
MICE
TRACER TECHNIQUES
ANIMALS
BACTERIA
BETA DECAY RADIOISOTOPES
CARBOHYDRATES
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LIPIDS
MAMMALS
MICROORGANISMS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
RADIOISOTOPES
REACTION KINETICS
RODENTS
SACCHARIDES
SEPARATION PROCESSES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques