CA V is present in rat kidney mitochondria
Guinea pig liver mitochondria contain the unique carbonic anhydrase isozyme, CA V. Prior to sacrifice, 15 rats and 15 guinea pigs were either fed normal lab chow (group 1), starved 48 hours (group 2) or fed normal lab chow and given to drink only water with added HCl, pH 2.5 (group 3). Mitochondria were prepared from excised livers and kidneys. CA V activity of disrupted mitochondria was measured by /sup 18/O-mass spectrometric technique at pH 7.4, 37/sup 0/C, 25 mM NaHCO/sub 3/. Mass spectrometric CA assays with intact kidney mitochondria localize CA V activity to the matrix, as was found for liver mitochondria. It has been shown in hepatocytes prepared from starved guinea pigs and rats that inhibition of CA V results in decreased rate of gluconeogenesis from pyruvate. These present results are in line with the published observation that rat kidneys are much more gluconeogenic than guinea pig, and that this is increased by starvation and acidosis.
- Research Organization:
- Univ. of Pennsylvania School of Medicine, Philadelphia
- OSTI ID:
- 6078365
- Report Number(s):
- CONF-870644-; TRN: 87-037189
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 46:6; Conference: 78. annual meeting of the American Society of Biological Chemists conference, Philadelphia, PA, USA, 7 Jun 1987
- Country of Publication:
- United States
- Language:
- English
Similar Records
Sequestration of /sup 45/Ca/sup 2 +/ by mitochondria from rabbit heart, liver, and kidney after doxorubicin or digoxin/doxorubicin treatment
Characterization of a new liver- and kidney-specific pfkfb3 isozyme that is downregulated by cell proliferation and dedifferentiation
Related Subjects
CARBONIC ANHYDRASE
BIOLOGICAL LOCALIZATION
ENZYME ACTIVITY
BIOLOGICAL VARIABILITY
FASTING
GUINEA PIGS
INHIBITION
KIDNEYS
LIVER
MASS SPECTROSCOPY
MITOCHONDRIA
OXYGEN 18
RATS
TRACER TECHNIQUES
ANIMALS
BODY
CARBON-OXYGEN LYASES
CELL CONSTITUENTS
DIGESTIVE SYSTEM
ENZYMES
EVEN-EVEN NUCLEI
GLANDS
HYDRO-LYASES
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
LYASES
MAMMALS
NUCLEI
ORGANOIDS
ORGANS
OXYGEN ISOTOPES
RODENTS
SPECTROSCOPY
STABLE ISOTOPES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques