Rapid changes in protein phosphorylation associated with gravity perception in corn roots. [Zea mays]
A previous paper from this laboratory showed calcium- and calmodulin-dependent in vivo protein phosphorylation in corn root tips. The authors show that rapid changes in calcium-dependent protein phosphorylation are involved in light-dependent graviperception in corn root tips. Corn seedlings (Zea mays L, cv Merit) were grown in the dark for 3 d, then apical root segments were harvested in dim green light to measure in vivo protein phosphorylation. Segments were incubated with 0.5 mCi /sup 32/P for 1 h, then immediately frozen in liquid N/sub 2/ or first treated with either 7 min light, or 7 min light plus 1 mM EGTA and 10 ..mu..M A23187. Labeled proteins were separated by 2D gel electrophoresis and detected by autoradiography. Light caused rapid and specific promotion of phosphorylation of 5 polypeptides. The increases in protein phosphorylation were reversed by treating with EGTA and A23187. The authors postulate that these changes in protein phosphorylation are an essential part of the light-dependent gravity response in Merit roots.
- Research Organization:
- Washington State Univ., Pullman
- OSTI ID:
- 6077891
- Report Number(s):
- CONF-8707108-; TRN: 87-037222
- Journal Information:
- Plant Physiol., Suppl.; (United States), Vol. 83:4; Conference: Annual meeting of the American Society of Plant Physiologists, St. Louis, MO, USA, 19 Jul 1987
- Country of Publication:
- United States
- Language:
- English
Similar Records
IAA transport in corn roots includes the root cap
Compartmentation of nucleotides in corn root tips studied by sup 31 P-NMR and HPLC. [Zea mays L]
Related Subjects
GRAVITATION
BIOLOGICAL EFFECTS
PROTEINS
PHOSPHORYLATION
VISIBLE SPECTRA
AUTORADIOGRAPHY
CALCIUM
ELECTROPHORESIS
LABELLED COMPOUNDS
MAIZE
PHOSPHORUS 32
ROOTS
SEEDLINGS
TRACER TECHNIQUES
ALKALINE EARTH METALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CEREALS
CHEMICAL REACTIONS
DAYS LIVING RADIOISOTOPES
ELEMENTS
GRASS
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
METALS
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHORUS ISOTOPES
PLANTS
RADIOISOTOPES
SPECTRA
550201* - Biochemistry- Tracer Techniques