Radiolocalization of bovine lymphosarcoma cells in athymic mice, using a monoclonal antibody against tumor-associated antigens

Journal Article · Sat Aug 01 00:00:00 EDT 1987 · Am. J. Vet. Res.; (United States)

OSTI ID:6075582

Aida, Y; Ochiai, K; Ito, K; Onuma, M; Fujimori, F; Fujimoto, Y; Izawa, H

Mouse monoclonal antibody c 143 was purified and F(ab')2 fragments were generated by pepsin digestion and then radiolabeled with /sup 125/I. The /sup 125/I-labeled c 143 F(ab')2 fragments were injected into athymic mice bearing bovine lymphoid tumor cells. The fragments became preferentially localized in tumor tissues, but not in normal tissues, as determined by differential counting of tissue radioactivity. The fragments became localized specifically in those tumors that were reactive with c 143 in vitro, but did not become localized in unrelated tumors. Localization of labeled F(ab')2 fragments of a monoclonal antibody of the same isotype directed against Taka virus (a variant of Newcastle disease virus) was not observed in athymic mice bearing bovine lymphoid tumor cells. Tumors were detectable by radioimmunoscintigraphy, using radiolabeled c 143 F(ab')2 fragments, without background subtraction, and by use of silver-grain scattering in light microscopic autoradiography.

• Research Organization: Hokkaido Univ., Sapporo, Japan
• OSTI ID: 6075582
• Journal Information: Am. J. Vet. Res.; (United States), Vol. 48:8
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
MONOCLONAL ANTIBODIES
BIOLOGICAL LOCALIZATION
LABELLING
SARCOMAS
DIAGNOSIS
ANTIGEN-ANTIBODY REACTIONS
AUTORADIOGRAPHY
CATTLE
IODINE 125
TUMOR CELLS
ANIMAL CELLS
ANIMALS
ANTIBODIES
BETA DECAY RADIOISOTOPES
DAYS LIVING RADIOISOTOPES
DISEASES
DOMESTIC ANIMALS
ELECTRON CAPTURE RADIOISOTOPES
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPES
MAMMALS
NEOPLASMS
NUCLEI
ODD-EVEN NUCLEI
RADIOISOTOPES
RUMINANTS
VERTEBRATES
550601* - Medicine- Unsealed Radionuclides in Diagnostics