Metabolism of monoterpanes: metabolic fate of (+)-camphor in sage (Salvia officinalis). [Salvia officinalis]

Journal Article · Wed Jul 01 00:00:00 EDT 1987 · Plant Physiol.; (United States)

OSTI ID:6075379

Croteau, R; El-Bialy, H; Dehal, S S

The bicyclic monoterpene ketone (+)-camphor undergoes lactonization to 1,2-campholide in mature sage (Salvia officinalis L.) leaves followed by conversion to the ..beta..-D-glucoside-6-O-glucose ester of the corresponding hydroxy acid (1-carboxymethyl-3-hydroxy-2,2,3-trimethyl cyclopentane). Analysis of the disposition of (+)-(G-/sup 3/H)camphor applied to midstem leaves of intact flowering plants allowed the kinetics of synthesis of the bis-glucose derivative and its transport from leaf to root to be determined, and gave strong indication that the transport derivative was subsequently metabolized in the root. Root extracts were shown to possess ..beta..-glucosidase and acyl glucose esterase activities, and studies with (+)-1,2(U-/sup 14/C)campholide as substrate, using excised root segments, revealed that the terpenoid was converted to lipid materials. Localization studies confirmed the radiolabeled lipids to reside in the membranous fractions of root extracts, and analysis of this material indicated the presence of labeled phytosterols and labeled fatty acids (C/sub 14/ to C/sub 20/) of acyl lipids. Although it was not possible to detail the metabolic steps between 1,2-campholide and the acyl lipids and phytosterols derived therefrom because of the lack of readily detectable intermediates, it seemed likely that the monoterpene lactone was degraded to acetyl CoA which was reincorporated into root membrane components via standard acyl lipid and isoprenoid biosynthetic pathways. Monoterpene catabolism thus appears to represent a salvage mechanism for recycling mobile carbon from senescing oil glands on the leaves to the roots.

• Research Organization: Washington State Univ., Pullman
• DOE Contract Number: AM06-76RL02221; AT06-82ER12027
• OSTI ID: 6075379
• Journal Information: Plant Physiol.; (United States), Vol. 84:3
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
LIPIDS
TRANSLOCATION
TERPENES
METABOLISM
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL LOCALIZATION
CAMPHOR
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CATABOLISM
CELL MEMBRANES
COENZYMES
ESTERASES
HERBS
HYDROLASES
LACTONES
LEAVES
METABOLITES
ORGANIC ACIDS
ROOTS
STEROLS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
CELL CONSTITUENTS
ENZYMES
ESTERS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
KETONES
KINETICS
LABELLED COMPOUNDS
MEMBRANES
ORGANIC COMPOUNDS
PLANTS
REACTION KINETICS
STEROIDS
550201* - Biochemistry- Tracer Techniques
551001 - Physiological Systems- Tracer Techniques