Excision repair of pyrimidine dimers induced by simulated solar radiation in the skin of patients with basal cell carcinoma
- Baylor College of Medicine, Houston, TX (USA)
One prominent lesion induced in DNA by ultraviolet (UV) radiation is the cyclobutyl pyrimidine dimer formed between adjacent pyrimidines on the same DNA strand. We investigated whether people who have developed basal cell carcinoma on sun-exposed skin have an altered ability to repair UV-induced pyrimidine dimers in DNA. Twenty-two patients with at least one basal cell carcinoma, aged 31-84 years, and 19 healthy volunteers, aged 25-61 years, took part in the study. Both groups were given one minimal erythema dose (MED) of simulated solar radiation on the lower back. DNA was extracted from the irradiated skin 0 to 6 h later, and the number of UV-induced pyrimidine dimers was determined using a dimer-specific endonuclease. At time 0, the average number of dimers per unit of DNA was similar in the two groups. After 6 h, an average of 22 +/- 4% of the dimers were removed in the group with basal cell carcinoma compared to 33 +/- 4% in the cancer-free group. In the basal cell carcinoma group, only 23% of the patients repaired more than 30% of the dimers after 6 h, compared with 53% of the cancer-free subjects (p less than 0.05). We conclude that patients who develop basal cell carcinoma on sun-exposed skin may have a decreased ability to repair pyrimidine dimers induced in skin exposed to simulated solar radiation.
- OSTI ID:
- 6074395
- Journal Information:
- Journal of Investigative Dermatology; (USA), Vol. 95:5; ISSN 0022-202X
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
PYRIMIDINE DIMERS
EXCISION REPAIR
SKIN
CARCINOMAS
DNA
DOSE-RESPONSE RELATIONSHIPS
ENDONUCLEASES
MAN
PATIENTS
RADIOINDUCTION
SIMULATION
ULTRAVIOLET RADIATION
ANIMALS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BODY
DISEASES
DNA REPAIR
DNA-ASE
ELECTROMAGNETIC RADIATION
ENZYMES
ESTERASES
HYDROLASES
MAMMALS
NEOPLASMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PHOSPHODIESTERASES
PRIMATES
RADIATIONS
RECOVERY
REPAIR
VERTEBRATES
550200* - Biochemistry
550900 - Pathology