Role of glutathione peroxidase in the radiation response of mouse kidney
Glutathione peroxidase (GSH-Px) has been implicated in mediating the radioprotective effects of glutathione (GSH). This hypothesis was tested in vivo by determining the effect of GSH-Px depletion on the radiation response of murine kidneys. Renal GSH-Px levels were depleted to 17% of control values by feeding animals a selenium deficient diet for 6 weeks; this had no significant effect on renal levels of GSH or GSH-S-transferase (GST). However, we also tested the effect of direct depletion of GSH to 3-4% of control values, using a combination of DL-buthionine sulphoximine (BSO) and diethyl maleate (DEM). Kidneys with normal or depleted levels of GSH-Px and/or GSH were irradiated with 240kVp X rays (2 fractions, 7 days apart to minimize intestinal injury). Mice breathed 7% oxygen during irradiation. Renal damage was assessed at 20, 25, and 32 weeks after the first fraction of X rays, in terms of reduced hematocrit and renal clearance of /sup 51/Cr-EDTA. Depletion of GSH-Px levels to 17% of control did not alter renal radiosensitivity, but depletion of GSH to 3-4% of control values radiosensitized the kidney by a factor of 1.4. Depletion of both GSH and GSH-Px together did not radiosensitize the kidney any more than was achieved by GSH depletion alone.
- Research Organization:
- Gray Laboratory of the Cancer Research Campaign, Middlesex (England)
- OSTI ID:
- 6073038
- Journal Information:
- Int. J. Radiat. Oncol., Biol. Phys.; (United States), Vol. 16:5
- Country of Publication:
- United States
- Language:
- English
Similar Records
Depletion of glutathione in vivo as a method of improving the therapeutic ratio of misonidazole and SR 2508. [BSO; DEM]
Radiation response of drug-resistant variants of a human breast cancer cell line: The effect of glutathione depletion
Related Subjects
59 BASIC BIOLOGICAL SCIENCES
KIDNEYS
RADIOSENSITIVITY
PEROXIDASES
RADIOSENSITIVITY EFFECTS
CHROMIUM 51
DIET
EDTA
FRACTIONATED IRRADIATION
MICE
TRACER TECHNIQUES
X RADIATION
AMINO ACIDS
ANIMALS
BETA DECAY RADIOISOTOPES
BODY
CARBOXYLIC ACIDS
CHELATING AGENTS
CHROMIUM ISOTOPES
ELECTROMAGNETIC RADIATION
ELECTRON CAPTURE RADIOISOTOPES
ENZYMES
EVEN-ODD NUCLEI
INTERMEDIATE MASS NUCLEI
IONIZING RADIATIONS
IRRADIATION
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
OXIDOREDUCTASES
RADIATIONS
RADIOISOTOPES
RODENTS
VERTEBRATES
560152* - Radiation Effects on Animals- Animals
550201 - Biochemistry- Tracer Techniques