Frequency of intrachromosomal homologous recombination induced by UV radiation in normally repairing and excision repair-deficient human cells
- Michigan State Univ., East Lansing (USA)
- Yale Univ. School of Medicine, New Haven, CT (USA)
To investigate the role of DNA damage and nucleotide excision repair in intrachromosomal homologous recombination, a plasmid containing duplicated copies of the gene coding for hygromycin resistance was introduced into the genome of a repair-proficient human cell line, KMST-6, and two repair-deficient lines, XP2OS(SV) from xeroderma pigmentosum complementation group A and XP2YO(SV) from complementation group F. Neither hygromycin-resistance gene codes for a functional enzyme because each contains an insertion/deletion mutation at a unique site, but recombination between the two defective genes can yield hygromycin-resistant cells. The rates of spontaneous recombination in normal and xeroderma pigmentosum cell strains containing the recombination substrate were found to be similar. The frequency of UV-induced recombination was determined for three of these cell strains. At low doses, the group A cell strain and the group F cell strain showed a significant increase in frequency of recombinants. The repair-proficient cell strain required 10-to 20-fold higher doses of UV to exhibit comparable increases in frequency of recombinants. These results suggest that unexcised DNA damage, rather than the excision repair process per se, stimulates such recombination.
- OSTI ID:
- 6068350
- Journal Information:
- Proceedings of the National Academy of Sciences of the United States of America; (USA), Vol. 87:4; ISSN 0027-8424
- Country of Publication:
- United States
- Language:
- English
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CHROMOSOMAL ABERRATIONS
RADIOINDUCTION
HETEROCHROMOSOMES
BIOLOGICAL REPAIR
XP CELLS
BIOLOGICAL RADIATION EFFECTS
DNA HYBRIDIZATION
DNA REPLICATION
EXTREME ULTRAVIOLET RADIATION
PHOSPHORUS 32
ANIMAL CELLS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL RECOVERY
CHROMOSOMES
DAYS LIVING RADIOISOTOPES
ELECTROMAGNETIC RADIATION
HYBRIDIZATION
ISOTOPES
LIGHT NUCLEI
MUTATIONS
NUCLEI
NUCLEIC ACID REPLICATION
ODD-ODD NUCLEI
PHOSPHORUS ISOTOPES
RADIATION EFFECTS
RADIATIONS
RADIOISOTOPES
RECOVERY
REPAIR
ULTRAVIOLET RADIATION
560120* - Radiation Effects on Biochemicals
Cells
& Tissue Culture