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Title: Glutamate and glycine modulation of 3H-MK801 binding to the NMDA receptor-ion channel complex in the vitamin B-6 deficient neonatal rat brain

The authors have previously shown that the concentrations of the neuroactive amino acids glutamate (GLU) and glycine (GLY) are significantly altered in the seizure-prone vitamin B-6 deficient neonatal rat brain. Recently, it has been shown that GLU and GLY modulate the binding of {sup 3}H-MK801 to the ion channel associated with the N-methyl-D-aspartate (NMDA)-glutamate receptor subtype. The present investigation was undertaken to determine if GLU or GLY modulation of {sup 3}H-MK801 binding was altered in B-6 deficient neonatal rat brain. Preparation of cortical membranes from control and deficient 14 day old rats and {sup 3}H-MK801 binding assay were done as described by Ransom and Stec. The results show a significant reduction in the potency and efficacy of GLU modulation of {sup 3}H-MK801 binding, as well as a reduction in the efficacy of GLY, in membrane preparations from deficient rats compared to controls. These results indicate a reduced ability of GLU and GLY to potentiate the binding of {sup 3}H-MK801 to the NMDA receptor-ion channel in the B-6 deficient neonatal rat brain.
Authors:
 [1]
  1. (Johns Hopkins Univ., Baltimore, MD (United States))
Publication Date:
OSTI Identifier:
6057291
Report Number(s):
CONF-9104107--
Journal ID: ISSN 0892-6638; CODEN: FAJOE
Resource Type:
Conference
Resource Relation:
Journal Name: FASEB Journal (Federation of American Societies for Experimental Biology); (United States); Journal Volume: 4:3; Conference: 75. annual meeting of the Federation of American Societies for Experimental Biology (FASEB), Atlanta, GA (United States), 21-25 Apr 1991
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; GLUTAMIC ACID; BIOLOGICAL EFFECTS; GLYCINE; PYRIDOXINE; BIOCHEMICAL REACTION KINETICS; BRAIN; NEONATES; NUTRITIONAL DEFICIENCY; RATS; RECEPTORS; TRACER TECHNIQUES; TRITIUM COMPOUNDS; AMINO ACIDS; ANIMALS; AZINES; BODY; CARBOXYLIC ACIDS; CENTRAL NERVOUS SYSTEM; HETEROCYCLIC COMPOUNDS; HYDROGEN COMPOUNDS; HYDROXY COMPOUNDS; ISOTOPE APPLICATIONS; KINETICS; MAMMALS; MEMBRANE PROTEINS; NERVOUS SYSTEM; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; ORGANS; PROTEINS; PYRIDINES; REACTION KINETICS; RODENTS; VERTEBRATES; VITAMIN B GROUP; VITAMINS 550201* -- Biochemistry-- Tracer Techniques