Effect of metals on water flow in glutaraldehyde fixed toad bladder

Conference · Mon Feb 26 00:00:00 EST 1990 · FASEB Journal (Federation of American Societies for Experimental Biology); (United States)

OSTI ID:6053884

Hoch, B S; Gorfien, P C; Eres, A; Shahmehdi, S; Lipner, H I ^[1]

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1. Maimonides Medical Center, Brooklyn, NY (United States)

The authors previously reported that HgCl{sub 2} inhibits flow in tissues fixed with glutaraldehyde after ADH stimulation. The authors examined the effect of numerous other metals on the ADH-induced water channel. Water flows were measured gravimetrically. In glutaraldehyde-fixed tissues water flow and sucrose permeability are maintained from pH 2.5 through pH 12. Several metals including Ba, Co, Fe, La, Sr, and Zn have no effect on flow. Al, Cd, Cu, Pb, and U all elicit a small but significant increase in water flow associated with inhibition of the increased sucrose permeability normally observed in glutaraldehyde-fixed tissues. Ag inhibits flow but increases sucrose permeability. Au inhibits flow by 90% in tissues fixed after pretreatment with ADH but increases the permeability of tissues fixed in the absence of ADH. These effects may result from disruption of epithelial integrity rather than direct blocking of the ADH-induced water channel. At pH {le} 3, Pt inhibits flow in ADH-pretreated tissues. Sucrose permeability is decreased and basal flow unaffected. No inhibitory effect is present at pH {ge} 3.5. The data indicate that at physiologic pH, the ADH induced water channel is blocked specifically by Hg but not by other metals.

• OSTI ID: 6053884
• Report Number(s): CONF-9104107-; CODEN: FAJOE
• Journal Information: FASEB Journal (Federation of American Societies for Experimental Biology); (United States), Vol. 4:3; Conference: 75. annual meeting of the Federation of American Societies for Experimental Biology (FASEB), Atlanta, GA (United States), 21-25 Apr 1991; ISSN 0892-6638
• Country of Publication: United States
• Language: English

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ALCOHOL DEHYDROGENASE
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ORGANIC COMPOUNDS
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