Induction of retrovirus gene expression in mouse cells by some chemical mutagens
Cell cultures derived from a variety of mouse strains were compared for their relative capacity to be induced to express endogenous retrovirus proteins by exposure to 5-iododeoxyuridine under optimized experimental conditions. Induction frequencies varied between 6.0 x 10/sup -1/ and 1.7 x 10/sup -2/ with AKR cells showing the highest capacity and C57BL/6 x C3H F/sub 1/ cells the lowest. Virus expression was induced in AKR cells with other chemical mutagens of the polyaromatic hydrocarbon (benzo(a)pyrene and phenol, diol, and epoxide metabolites) and nucleoside analog classes, but alkylating agents were inconsistent or failed to induce. Considerable differences in the efficiency of induction were seen between various halogenated nucleosides, while under these conditions the nucleoside 5-azacytidine induced > 90% of AKR cells at a concentration of 2 to 4 ..mu..g/ml. The high frequency of induction by 5-azacytidine, relative to other nucleoside analogs, and the absence of induction by other mutgens further indicate that endogenous virus induction occurs via nonmutagenic mechanisms and that some mutagens may also affect regulatory functions independent of their mutagenic action.
- Research Organization:
- Oak Ridge National Lab., TN
- DOE Contract Number:
- W-7405-ENG-26
- OSTI ID:
- 6047359
- Journal Information:
- Cancer Res.; (United States), Vol. 42
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
BENZOPYRENE
BIOLOGICAL EFFECTS
EPOXIDES
GLYCOLS
IODODEOXYURIDINE
ORGANIC NITROGEN COMPOUNDS
PHENOL
VIRUSES
STIMULATION
ALKYLATING AGENTS
ANIMAL CELLS
CELL CULTURES
CYTIDINE
EXPERIMENTAL DATA
GENETICS
MICE
NUCLEOSIDES
PLAQUE FORMATION
ALCOHOLS
ANIMALS
ANTIMETABOLITES
AROMATICS
AZINES
BIOLOGY
CONDENSED AROMATICS
DATA
DRUGS
HETEROCYCLIC COMPOUNDS
HYDROCARBONS
HYDROXY COMPOUNDS
INFORMATION
IODOURACILS
MAMMALS
MICROORGANISMS
NUCLEOTIDES
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC IODINE COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
PARASITES
PHENOLS
PYRIMIDINES
RIBOSIDES
RODENTS
URACILS
VERTEBRATES
560301* - Chemicals Metabolism & Toxicology- Cells- (-1987)
560305 - Chemicals Metabolism & Toxicology- Vertebrates- (-1987)