Biochemical characterization of domain-specific glycoproteins of the rat hepatocyte plasma membrane
Seven integral proteins (CE 9, HA 21, HA 116, HA 16, HA 4, HA 201, and HA 301) were isolated from rat hepatocyte plasma membranes by immunoaffinity chromatography on monoclonal antibody-Sepharose. Six of the proteins (all but HA 16) exhibit domain-specific localizations (either bile canalicular or sinusoidal/lateral) about the hepatocyte surface. The authors identified three of these protein antigens as leucine aminopeptidase (HA 201), dipeptidyl peptidase IV (HA 301), and the asialoglycoprotein receptor (HA 116). They also developed SVI-lectin blotting procedures that, when used in conjunction with chemical and glycosidase treatments, permitted a comparison of the types of oligosaccharides present on the seven proteins. All seven are sialoglycoproteins, based upon the effects of prior neuraminidase and periodate-aniline-cyanoborohydride treatments of blots on labeling by SVI-wheat germ agglutinin. Depending upon the protein, they estimated the presence of 2-26 N-linked oligosaccharides/polypeptide chain from the Mr reductions accompanying chemical or enzymatic deglycosylation. Three of these mature plasma membrane proteins (HA 21, HA 116, and HA 4) have both high mannose-type and complex-type oligosaccharides on every copy of their polypeptide chains.
- Research Organization:
- Johns Hopkins Univ. School of Medicine, Baltimore, MD
- OSTI ID:
- 6044954
- Journal Information:
- J. Biol. Chem.; (United States), Vol. 23
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CELL MEMBRANES
MOLECULAR STRUCTURE
GLUCOPROTEINS
BIOCHEMISTRY
IODINE 125
TRACER TECHNIQUES
AMINOPEPTIDASES
ANTIGENS
LECTINS
LIVER CELLS
MANNOSE
MICE
MONOCLONAL ANTIBODIES
OLIGOSACCHARIDES
RATS
RECEPTORS
ALDEHYDES
ANIMAL CELLS
ANIMALS
ANTIBODIES
BETA DECAY RADIOISOTOPES
CARBOHYDRATES
CELL CONSTITUENTS
CHEMISTRY
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
ENZYMES
HEXOSES
HYDROLASES
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MEMBRANES
MONOSACCHARIDES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PEPTIDE HYDROLASES
PROTEINS
RADIOISOTOPES
RODENTS
SACCHARIDES
SOMATIC CELLS
VERTEBRATES
550301* - Cytology- Tracer Techniques
550201 - Biochemistry- Tracer Techniques