Biotransformation of primary nicotine metabolites. I. In vivo metabolism of R-(+)-( UC-NCH3)N-methylnicotinium ion in the guinea pig
The in vivo biotransformation and tissue distribution of the methylated nicotine metabolite R-(+)-( UC-NCH3)N-methylnicotinium acetate was studied in the guinea pig. The detection and quantification of 24-hr urinary metabolites after ip injection was determined by cation-exchange HPLC interfaced to a radiochemical flowthrough detector. The urinary metabolite profile consisted of five peaks. One eluted close to the void, and three coeluted with authentic standards of N-methylcotininium ion, N-methylnornicotinium ion, and N-methylnicotinium ion. A fifth, and as yet unidentified, metabolite was also detected. Tissue distribution of UC label after 24 hr was highest in the adrenal gland and epididymis followed by the gallbladder, bladder, kidney, spleen, and heart. No significant amounts of UC were found in the brain. The results indicate that N-methylcotininium ion and N-methylnornicotinium ion are both formed subsequent to the formation of N-methylnicotinium ion in the metabolism of R-(+)-nicotine in the guinea pig.
- Research Organization:
- Univ. of Kentucky, Lexington
- OSTI ID:
- 6044454
- Journal Information:
- Drug Metab. Dispos.; (United States), Vol. 5
- Country of Publication:
- United States
- Language:
- English
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CARBON 14 COMPOUNDS
TISSUE DISTRIBUTION
NICOTINE
ACETATES
ADRENAL GLANDS
BILIARY TRACT
BLADDER
GUINEA PIGS
HEART
KIDNEYS
LIQUID COLUMN CHROMATOGRAPHY
METABOLITES
METHYLATION
SPLEEN
ALKALOIDS
AMINES
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
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AZOLES
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CARBOXYLIC ACID SALTS
CARDIOVASCULAR SYSTEM
CHEMICAL REACTIONS
CHROMATOGRAPHY
DIGESTIVE SYSTEM
DISTRIBUTION
DRUGS
ENDOCRINE GLANDS
GLANDS
HETEROCYCLIC COMPOUNDS
LABELLED COMPOUNDS
MAMMALS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PARASYMPATHOLYTICS
PARASYMPATHOMIMETICS
PYRIDINES
PYRROLES
PYRROLIDINES
RODENTS
SEPARATION PROCESSES
URINARY TRACT
VERTEBRATES
550501* - Metabolism- Tracer Techniques