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Title: NMR studies of renal phosphate metabolites in vivo: Effects of hydration and dehydration

The present study characterizes the {sup 31}P-nuclear magnetic resonance (NMR) spectrum of rabbit kidneys in vivo and evaluates the effect of hydration on phosphorous metabolites including the organic solute glycerophosphorylcholine (GPC). Cortical phosphorylethanolamine is the predominant component of the phosphomonoester region of the {sup 31}P spectrum. The contribution of blood to the spectrum is mainly from 2,3 diphosphoglycerate, which comprises {approximately}30% of the inorganic phosphate region. Acute infusion of 0.9% saline decreases the sodium content of the inner medulla by >50% in 15 min as shown by {sup 23}Na imaging. Despite this medullary Na dilution, no change in renal GPC content was observed for >1 h even with the addition of furosemide or furosemide and antidiuretic hormone. However, 20 h of chronic dehydration with 0.45% saline did result in a 30% decrease in renal GPC content when compared with dehydrated animals. These findings are consistent with GPC not playing a role in the short-term regulation of the medullary intracellular milieu in response to acute reductions in medullary Na content.
Authors:
; ;  [1]
  1. (National Institutes of Health, Bethesda, MD (USA))
Publication Date:
OSTI Identifier:
6038124
Resource Type:
Journal Article
Resource Relation:
Journal Name: American Journal of Physiology; (USA); Journal Volume: 255:4
Country of Publication:
United States
Language:
English
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; PHOSPHOLIPIDS; NUCLEAR MAGNETIC RESONANCE; CHOLINE; DEHYDRATION; HYDRATION; IN VIVO; INOSITOLS; KIDNEYS; METABOLITES; PHOSPHORUS 31; RABBITS; ALCOHOLS; AMINES; AMMONIUM COMPOUNDS; ANIMALS; BODY; CARBOHYDRATES; DRUGS; ESTERS; HYDROXY COMPOUNDS; ISOTOPES; LIGHT NUCLEI; LIPIDS; LIPOTROPIC FACTORS; MAGNETIC RESONANCE; MAMMALS; MONOSACCHARIDES; NUCLEI; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; ORGANIC PHOSPHORUS COMPOUNDS; ORGANS; PHOSPHORUS ISOTOPES; QUATERNARY COMPOUNDS; RESONANCE; SACCHARIDES; SOLVATION; STABLE ISOTOPES; VERTEBRATES 550601* -- Medicine-- Unsealed Radionuclides in Diagnostics