Pathway of assembly of ribulosebisphosphate carboxylase/oxygenase from Anabaena 7210 expressed in Escherichia coli

Journal Article · Tue Oct 01 00:00:00 EDT 1985 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.82.19.6546· OSTI ID:6038055

Gurevitz, M; Somerville, C R; McIntosh, L

The authors have placed the genes encoding ribulosebisphosphate carboxylase/oxygenase from the Anabaena 7120 operon under transcriptional control of the lac promoter carried on the Escherichia coli plasmid pUC19. The genes encoding both the large and small subunit polypeptides (rbcL and rbcS) are transcribed and translated so that approx. = 0.6% of the soluble protein in E. coli extracts is a fully functional holoenzyme with a sedimentation coefficient of approximately 18S, which contains stoichiometric amounts of the two subunits. However, expression of the large subunit polypeptide vastly exceeds that of the small subunit because the majority of transcripts terminate in the intergenic region between the rbcL and rbcS genes. As a result, excess large subunit is synthesized and accumulates in E. coli as an insoluble and catalytically inactive form. Because small subunit is found only in the high molecular weight soluble form of ribulosebisphosphate carboxylase/oxygenase, the authors propose that the small subunit promotes assembly of the hexadecameric form of the enzyme via heterodimers of large and small subunits.

• Research Organization: Michigan State Univ., East Lansing, MI (United States)
• DOE Contract Number: AC02-76ER01338
• OSTI ID: 6038055
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 82:19
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CARBOXYLASE
BIOLOGICAL PATHWAYS
OXYGENASES
CYANOBACTERIA
ESCHERICHIA COLI
GENES
MOLECULAR WEIGHT
PLASMIDS
POLYPEPTIDES
TRANSCRIPTION
BACTERIA
CARBON-CARBON LYASES
CARBOXY-LYASES
CELL CONSTITUENTS
ENZYMES
LYASES
MICROORGANISMS
ORGANIC COMPOUNDS
OXIDOREDUCTASES
PEPTIDES
PROTEINS
550200* - Biochemistry