The effects of drugs, other foreign compounds, and cigarette smoke on the synthesis of protein by lung slices

Journal Article · Sun Apr 01 00:00:00 EST 1990 · Environmental Health Perspectives; (USA)

DOI:https://doi.org/10.2307/3430677· OSTI ID:6034471

Hellstern, K; Curtis, C G; Powell, G M ^[1]; Upshall, D G ^[2]

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1. University College, Cardiff (England)
2. Chemical Defence Establishment, Wiltshire (England)

The incorporation of {sup 14}C-leucine into rabbit lung slices was monitored in the absence and presence of selected drugs and chemicals relevant to the perturbation of lung function and the development of lung disease. Known inhibitors of protein synthesis (cycloheximide and ricin) inhibited the incorporation of {sup 14}C-leucine. Marked inhibition was also recorded with the lung toxins paraquat and 4-ipomeanol. By contrast, orciprenaline, salbutamol, and terbutaline were without effect although some response was recorded with isoprenaline. The filtered gas phase of cigarette smoke and acrolein, one of its components, were inhibitory but protection was afforded by N-acetylcysteine. It is suggested that the inhibitory effects of cigarette smoke may be due to its acrolein content. It is further suggested that the use of lung slices and measurements of {sup 14}C-leucine incorporation provide valuable means for monitoring potential pulmonary toxins.

• OSTI ID: 6034471
• Journal Information: Environmental Health Perspectives; (USA), Vol. 85; ISSN 0091-6765
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
62 RADIOLOGY AND NUCLEAR MEDICINE
BIPYRIDINES
BIOLOGICAL EFFECTS
CARBON 14 COMPOUNDS
UPTAKE
PROTEINS
BIOSYNTHESIS
TOBACCO SMOKES
ACROLEIN
CYCLOHEXIMIDE
LEUCINE
LUNGS
RABBITS
AEROSOLS
ALDEHYDES
AMINO ACIDS
ANIMALS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
AZINES
BODY
CARBOXYLIC ACIDS
COLLOIDS
DISPERSIONS
DRUGS
FUNGICIDES
HETEROCYCLIC COMPOUNDS
LABELLED COMPOUNDS
MAMMALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PESTICIDES
PYRIDINES
RESIDUES
RESPIRATORY SYSTEM
SMOKES
SOLS
SYNTHESIS
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology
550601 - Medicine- Unsealed Radionuclides in Diagnostics