Ozone stress induces the expression of ACC synthase in potato plants

Conference · Sat May 01 00:00:00 EDT 1993 · Plant Physiology; (United States)

OSTI ID:6028383

Schlagnhaufer, C D; Arteca, R N; Pell, E J ^[1]

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1. Pennsylvania State Univ., University Park (United States)

When potato plants (Solanum tuberosum L. cv Norland) are subjected to oxone stress ethylene is emitted. Increases in ethylene production are often the result of increased expression of the enzyme ACC synthase. We used the polymerase chain reaction (PCR) to clone a cDNA encoding an ozone-induced ACC synthase. After treating potato plants with 300 ppb ozone for 4 h, RNA was extracted using a guanidinium isothiocyanate method. Using degenerate oligonucleotides corresponding to several conserved regions of ACC synthase sequences reported from different plant tissues as primers, we were able to reverse transcribe the RNA and amplify a cDNA for ACC synthase. The clone is 1098 bp in length encoding for 386 amino acids comprising [approximately]80% of the protein. Computer analysis of the deduced amino acid sequence showed that our clone is 50-70% homologous with ACC synthase genes cloned from other plant tissues. Using the cDNA as a probe in northern analysis we found that there is little or no expression in control tissue: however there is a large increase in the expression of the ACC synthase message in response to ozone treatment.

• OSTI ID: 6028383
• Report Number(s): CONF-9307119-; CODEN: PLPHAY
• Journal Information: Plant Physiology; (United States), Vol. 102:1; Conference: 1993 joint annual meeting of the American Society of Plant Physiologists (ASPP) and the Canadian Society of Plant Physiologists (CSPP), Minneapolis, MN (United States), 31 Jul - 4 Aug 1993; ISSN 0032-0889
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ENZYMES
ECOLOGICAL CONCENTRATION
OZONE
BIOLOGICAL EFFECTS
SOLANUM TUBEROSUM
AMINO ACID SEQUENCE
DNA-CLONING
ETHYLENE
GENES
PLANTS
ALKENES
CLONING
DNA HYBRIDIZATION
HYBRIDIZATION
HYDROCARBONS
MAGNOLIOPHYTA
MAGNOLIOPSIDA
MOLECULAR STRUCTURE
ORGANIC COMPOUNDS
PROTEINS
SOLANUM
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