Radioenzymatic microassay for picogram quantities of serotonin or acetylserotonin in biological fluids and tissues

Journal Article · Mon Jun 01 00:00:00 EDT 1987 · Biochem. Med. Metab. Biol.; (United States)

DOI:https://doi.org/10.1016/0885-4505(87)90042-9· OSTI ID:6024865

Hussain, M N; Benedict, C R

This paper describes several modifications of the original radioenzymatic assay for serotonin which increase the sensitivity of the assay 20-fold as well as enhance its reliability. Using this method serotonin concentrations can be directly measured in biological examples without precleaning the sample. When compared to currently available methods this assay is specific and sensitive to approximately 1 pg of serotonin and can be used to measure serotonin levels in individual brain nuclei or microliter quantities of biological fluids. This assay can be easily adapted for the direct measurement of N-acetylserotonin. A large number of samples can be assayed in a single working day.

• Research Organization: Univ. of Texas Medical Branch, Galveston
• OSTI ID: 6024865
• Journal Information: Biochem. Med. Metab. Biol.; (United States), Vol. 37:3
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
RADIOENZYMATIC ASSAY
SENSITIVITY ANALYSIS
SEROTONIN
ANIMAL TISSUES
BODY FLUIDS
BRAIN
CELL NUCLEI
ERYTHROCYTES
IMIPRAMINE
TRITIUM COMPOUNDS
AMINES
ANTIDEPRESSANTS
AROMATICS
AUTONOMIC NERVOUS SYSTEM AGENTS
AZAARENES
AZOLES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
CELL CONSTITUENTS
CENTRAL NERVOUS SYSTEM
CENTRAL NERVOUS SYSTEM AGENTS
DRUGS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
INDOLES
LABELLED COMPOUNDS
MATERIALS
NERVOUS SYSTEM
NEUROREGULATORS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PSYCHOTROPIC DRUGS
PYRROLES
RADIOPROTECTIVE SUBSTANCES
SYMPATHOMIMETICS
TISSUES
TRYPTAMINES
550201* - Biochemistry- Tracer Techniques