Trafficking of. cap alpha. -L-fucosidase in lymphoid cells

DiCioccio, R A; Brown, K S

Title: Trafficking of. cap alpha. -L-fucosidase in lymphoid cells

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Abstract

The quantity of ..cap alpha..-L-fucosidase in human serum is determined by heredity. The mechanism controlling levels of the enzyme in serum is unknown. To investigate this, lymphoid cell lines derived from individuals with either low, intermediate or high ..cap alpha..-L-fucosidase in serum were established. Steady state levels of extracellular ..cap alpha..-L-fucosidase protein and activity overlapped among the cell lines. Thus, in vivo serum phenotypes of ..cap alpha..-L-fucosidase are not adequately expressed in this system. ..cap alpha..-L-Fucosidase was also metabolically labelled with /sup 35/S-methionine, immunoprecipitated, and examined by SDS-PAGE. Cells pulse-labelled from 0.25-2 h had a major intracellular form of enzyme (Mr = 58,000). Cells pulsed for 1.5 h and chased for 21 h with unlabeled methionine had an intracellular form of Mr = 60,000 and an extracellular form of Mr = 62,000. Cells treated with chloroquine had only the 58,000-form both intra- and extra-cellularly. Moreover, chloroquine did not effect the quantitative distribution of ..cap alpha..-L-fucosidase between cells and medium. In fibroblasts, chloroquine enhanced the secretion of newly made lysosomal enzymes and blocked the processing of intercellular enzyme forms from a higher to a lower molecular mass. Thus, there are trafficking differences between ..cap alpha..-L-fucosidase in lymphoid cells and lysosomal enzymesmore »« less

Authors:: DiCioccio, R A; Brown, K S

Publication Date:: Fri May 01 00:00:00 EDT 1987

Research Org.:: Roswell Park Memorial Institute, Buffalo, NY

OSTI Identifier:: 6024582

Report Number(s):: CONF-870644-
Journal ID: CODEN: FEPRA; TRN: 87-033977

Resource Type:: Conference

Journal Name:: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

Additional Journal Information:: Journal Volume: 46:6; Conference: 78. annual meeting of the American Society of Biological Chemists conference, Philadelphia, PA, USA, 7 Jun 1987

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES; GLYCOSYL HYDROLASES; ENZYME ACTIVITY; SECRETION; ELECTROPHORESIS; FIBROBLASTS; LYMPHOCYTES; LYSOSOMES; MAN; METHIONINE; SULFUR 35; TRACER TECHNIQUES; AMINO ACIDS; ANIMAL CELLS; ANIMALS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY FLUIDS; CARBOXYLIC ACIDS; CELL CONSTITUENTS; CONNECTIVE TISSUE CELLS; DAYS LIVING RADIOISOTOPES; DRUGS; ENZYMES; EVEN-ODD NUCLEI; HYDROLASES; ISOTOPE APPLICATIONS; ISOTOPES; LEUKOCYTES; LIGHT NUCLEI; LIPOTROPIC FACTORS; MAMMALS; MATERIALS; NUCLEI; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC SULFUR COMPOUNDS; ORGANOIDS; PRIMATES; RADIOISOTOPES; SOMATIC CELLS; SULFUR ISOTOPES; VERTEBRATES; 550201* - Biochemistry- Tracer Techniques

Citation Formats


                    DiCioccio, R A, and Brown, K S. Trafficking of. cap alpha. -L-fucosidase in lymphoid cells.  United States: N. p., 1987. 
        Web.

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                    DiCioccio, R A, & Brown, K S. Trafficking of. cap alpha. -L-fucosidase in lymphoid cells.  United States.

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                    DiCioccio, R A, and Brown, K S. 1987.  
        "Trafficking of. cap alpha. -L-fucosidase in lymphoid cells".  United States.

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@article{osti_6024582,

  title        = {Trafficking of. cap alpha. -L-fucosidase in lymphoid cells},

  author       = {DiCioccio, R A and Brown, K S},

  abstractNote = {The quantity of ..cap alpha..-L-fucosidase in human serum is determined by heredity. The mechanism controlling levels of the enzyme in serum is unknown. To investigate this, lymphoid cell lines derived from individuals with either low, intermediate or high ..cap alpha..-L-fucosidase in serum were established. Steady state levels of extracellular ..cap alpha..-L-fucosidase protein and activity overlapped among the cell lines. Thus, in vivo serum phenotypes of ..cap alpha..-L-fucosidase are not adequately expressed in this system. ..cap alpha..-L-Fucosidase was also metabolically labelled with /sup 35/S-methionine, immunoprecipitated, and examined by SDS-PAGE. Cells pulse-labelled from 0.25-2 h had a major intracellular form of enzyme (Mr = 58,000). Cells pulsed for 1.5 h and chased for 21 h with unlabeled methionine had an intracellular form of Mr = 60,000 and an extracellular form of Mr = 62,000. Cells treated with chloroquine had only the 58,000-form both intra- and extra-cellularly. Moreover, chloroquine did not effect the quantitative distribution of ..cap alpha..-L-fucosidase between cells and medium. In fibroblasts, chloroquine enhanced the secretion of newly made lysosomal enzymes and blocked the processing of intercellular enzyme forms from a higher to a lower molecular mass. Thus, there are trafficking differences between ..cap alpha..-L-fucosidase in lymphoid cells and lysosomal enzymes in fibroblasts. This suggests that alternative targeting mechanisms for lysosomal enzymes exist in these cells.},

  doi          = {},

  url          = {https://www.osti.gov/biblio/6024582},
  journal      = {Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)},
number       = ,

  volume       = 46:6,

  place        = {United States},

  year         = {Fri May 01 00:00:00 EDT 1987},

  month        = {Fri May 01 00:00:00 EDT 1987}

}

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