Radiosensitization by misonidazole during recovery of cellular thiols following depletion by BSO or DEM

Journal Article · Mon May 01 00:00:00 EDT 1989 · Int. J. Radiat. Oncol., Biol. Phys.; (United States)

DOI:https://doi.org/10.1016/0360-3016(89)90309-X· OSTI ID:6024248

McNally, N J; Soranson, J A

V79 cells have been depleted of their endogenous thiols by treatment with 100 microM BSO for 16-18 hr, or 0.5 mM DEM for 1 hr. The recovery of cellular thiols after removal of the drugs was determined by h.p.l.c. or flow cytometry and the sensitizer enhancement ratio for 100 microM misonidazole was measured as a function of time after removal of the drugs. The SER of 1.2 for control (hypoxic) cells increased to 1.8 for BSO treated (hypoxic) cells and 2.2 for DEM treated ones, when thiol levels were below 10% of controls. The SER and thiol levels returned to control values within 5 hr of removing DEM. After BSO there was little change during the first 5 hr and then a gradual return to normal values by 24 hrs. The major fall in the SER after removal of the drugs occurred as the cellular thiols increased to 60% of control values.

• Research Organization: Cancer Research Campaign Gray Laboratory, Mount Vernon Hospital, Northwood, Middlesex (England)
• OSTI ID: 6024248
• Journal Information: Int. J. Radiat. Oncol., Biol. Phys.; (United States), Vol. 16:5
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
MISONIDAZOLE
RADIOSENSITIVITY EFFECTS
ANOXIA
CELL FLOW SYSTEMS
FIBROBLASTS
GLUTATHIONE
HAMSTERS
LIQUID COLUMN CHROMATOGRAPHY
LUNGS
METHIONINE
SURVIVAL CURVES
AMINO ACIDS
ANIMAL CELLS
ANIMALS
ANTINEOPLASTIC DRUGS
AZOLES
BODY
CARBOXYLIC ACIDS
CHROMATOGRAPHY
CONNECTIVE TISSUE CELLS
DRUGS
HETEROCYCLIC COMPOUNDS
IMIDAZOLES
LIPOTROPIC FACTORS
MAMMALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
PEPTIDES
POLYPEPTIDES
PROTEINS
RADIOPROTECTIVE SUBSTANCES
RADIOSENSITIZERS
RESPIRATORY SYSTEM
RODENTS
SEPARATION PROCESSES
SOMATIC CELLS
VERTEBRATES
560120* - Radiation Effects on Biochemicals
Cells
& Tissue Culture