Toxicity of petroleum crude oils and their effect on xenobiotic metabolizing enzyme activities in the chicken embryo in ovo
Abstract
Microliter quantities of a Prudhoe Bay crude oil (PBCO) applied to the shell of fertile chick eggs during various stages of development induced cytochrome P-450 levels and mixed-function oxidase activities within the liver of the embryo. PBCO (5 ..mu..l) applied on Day 11 of incubation was found to maximally induce within 24 hr embryo hepatic cytochrome P-450 levels (fourfold), naphthalene hydroxylase (sixfold), benzo(a)pyrene 3-hyroxylase (14-fold), and 7-ethoxyresorufin O-deethylase (24-fold). Glutathione S-transferase was not induced. Crude oils are known to be highly toxic to avian embryos, especially during the early stages of development. The LD/sub 50/ of PBCO and Hibernia crude oil applied to the egg shell on Day 8 of incubation was found to be 1.3 and 2.2 ..mu..l, respectively. Mixed-function oxidase-dependent metabolism of crude oil components may be required for toxicity since administration of 20 ..mu..g of disulfiram in dioxane 1 hr prior to application of 1.3 ..mu..l of PBCO reduced embryo mortality from 60 to 20%.
- Authors:
- Publication Date:
- Research Org.:
- Memorial Univ. of Newfoundland, St. John's
- OSTI Identifier:
- 6023472
- Resource Type:
- Journal Article
- Journal Name:
- Environ. Res.; (United States)
- Additional Journal Information:
- Journal Volume: 39:1
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; 02 PETROLEUM; CYTOCHROME OXIDASE; ENZYME ACTIVITY; EMBRYOS; SENSITIVITY; HYDROXYLASES; MIXED-FUNCTION OXIDASES; PETROLEUM; TOXICITY; XENOBIOTICS; METABOLISM; CHICKENS; ANIMALS; BIRDS; ENERGY SOURCES; ENZYMES; FOSSIL FUELS; FOWL; FUELS; HAEM DEHYDROGENASES; OXIDOREDUCTASES; OXYGENASES; VERTEBRATES; 560305* - Chemicals Metabolism & Toxicology- Vertebrates- (-1987); 020900 - Petroleum- Environmental Aspects
Citation Formats
Lee, Y Z, O'Brien, P J, Payne, J F, and Rahimtula, A D. Toxicity of petroleum crude oils and their effect on xenobiotic metabolizing enzyme activities in the chicken embryo in ovo. United States: N. p., 1986.
Web. doi:10.1016/S0013-9351(86)80017-2.
Lee, Y Z, O'Brien, P J, Payne, J F, & Rahimtula, A D. Toxicity of petroleum crude oils and their effect on xenobiotic metabolizing enzyme activities in the chicken embryo in ovo. United States. https://doi.org/10.1016/S0013-9351(86)80017-2
Lee, Y Z, O'Brien, P J, Payne, J F, and Rahimtula, A D. 1986.
"Toxicity of petroleum crude oils and their effect on xenobiotic metabolizing enzyme activities in the chicken embryo in ovo". United States. https://doi.org/10.1016/S0013-9351(86)80017-2.
@article{osti_6023472,
title = {Toxicity of petroleum crude oils and their effect on xenobiotic metabolizing enzyme activities in the chicken embryo in ovo},
author = {Lee, Y Z and O'Brien, P J and Payne, J F and Rahimtula, A D},
abstractNote = {Microliter quantities of a Prudhoe Bay crude oil (PBCO) applied to the shell of fertile chick eggs during various stages of development induced cytochrome P-450 levels and mixed-function oxidase activities within the liver of the embryo. PBCO (5 ..mu..l) applied on Day 11 of incubation was found to maximally induce within 24 hr embryo hepatic cytochrome P-450 levels (fourfold), naphthalene hydroxylase (sixfold), benzo(a)pyrene 3-hyroxylase (14-fold), and 7-ethoxyresorufin O-deethylase (24-fold). Glutathione S-transferase was not induced. Crude oils are known to be highly toxic to avian embryos, especially during the early stages of development. The LD/sub 50/ of PBCO and Hibernia crude oil applied to the egg shell on Day 8 of incubation was found to be 1.3 and 2.2 ..mu..l, respectively. Mixed-function oxidase-dependent metabolism of crude oil components may be required for toxicity since administration of 20 ..mu..g of disulfiram in dioxane 1 hr prior to application of 1.3 ..mu..l of PBCO reduced embryo mortality from 60 to 20%.},
doi = {10.1016/S0013-9351(86)80017-2},
url = {https://www.osti.gov/biblio/6023472},
journal = {Environ. Res.; (United States)},
number = ,
volume = 39:1,
place = {United States},
year = {Wed Jan 01 00:00:00 EST 1986},
month = {Wed Jan 01 00:00:00 EST 1986}
}