Contribution of vitamin K1 to the electron spin polarization in spinach photosystem I

Journal Article · Tue Sep 04 00:00:00 EDT 1990 · Biochemistry; (USA)

DOI:https://doi.org/10.1021/bi00487a006· OSTI ID:6014896

Rustandi, R R; Snyder, S W; Feezel, L L; Michalski, T J; Norris, J R; Thurnauer, M C; Biggins, J ^[1]

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1. Argonne National Laboratory, IL (USA)

The electron spin polarized (ESP) electron paramagnetic resonance (EPR) signal observed in spinach photosystem I (PSI) particles was examined in preparations depleted of vitamin K1 by solvent extraction and following biological reconstitution by the quinone. The ESP EPR signal was not detected in the solvent-extracted PSI sample but was restored upon reconstitution with either protonated or deuterated vitamin K1 under conditions that also restored electron transfer to the terminal PSI acceptors. Reconstitution using deuterated vitamin K1 resulted in a line narrowing of the ESP EPR signal, supporting the conclusion that the ESP EPR signals in the reconstituted samples arise from a radical pair consisting of the oxidized PSI primary donor, P700+, and reduced vitamin K1.

• OSTI ID: 6014896
• Journal Information: Biochemistry; (USA), Vol. 29:35; ISSN 0006-2960
• Country of Publication: United States
• Language: English

Related Subjects

14 SOLAR ENERGY
59 BASIC BIOLOGICAL SCIENCES
PHOTOSYNTHETIC REACTION CENTERS
ELECTRON SPIN RESONANCE
VITAMIN K
BIOLOGICAL FUNCTIONS
DEUTERIUM
ELECTRON TRANSFER
REDOX REACTIONS
SOLVENT EXTRACTION
SPINACH
TRACER TECHNIQUES
AROMATICS
CHEMICAL REACTIONS
COAGULANTS
DRUGS
EXTRACTION
FOOD
FUNCTIONS
HEMATOLOGIC AGENTS
HEPARIN ANTAGONISTS
HYDROGEN ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MAGNETIC RESONANCE
MAGNOLIOPHYTA
MAGNOLIOPSIDA
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
PLANTS
QUINONES
RESONANCE
SEPARATION PROCESSES
STABLE ISOTOPES
VEGETABLES
VITAMINS
140505* - Solar Energy Conversion- Photochemical
Photobiological
& Thermochemical Conversion- (1980-)
550201 - Biochemistry- Tracer Techniques