Influences of soil acidity and variety of plant on the populations of Rhizobium trifolii found in nodules of Trifolium subterraneum L

Thesis/Dissertation · Sun Jan 01 00:00:00 EST 1984

OSTI ID:6009662

Dughri, M H

Complementary methods of strain identification were sought to delineate the composition of the populations of Rhizobium trifoli found in nodules of sub-clover when exposed to soil containing a resident population of R. trifolii. Polyacrylamide gel electrophoresis (PAGE) was used in a microslab system to elucidate the protein profile patterns of the isolates. Antisera were raised to four isolates which showed distinctly different protein profile patterns. Serological tests showed that the majority of nodule isolates could be placed into four serogroups, isolates within two of the groups (16 and 36), were antigenically identical whereas isolates in the other two groups (6 and 27) were antigenically heterogeneous. PAGE revealed that antigenically identical isolates could be subdivided further. Soil acidity affected which members of the indigenous population nodulated T. subterraneum L. Representatives of serogroup 6 occupied the greatest percentage of the nodules formed on plants grown at low pH and were a minor nodule occupant at the higher pH. In contrast sergroup 36 was virtually absent in nodules formed at low pH and was a dominant serogroup at the higher pH. Despite the isolates within serogroups 6 and 36 being antigenically identical separation of cellular proteins by PAGE revealed that serogroups 6 and 36 were composed minimally of eight and twelve different strains.

• OSTI ID: 6009662
• Resource Relation: Other Information: Thesis (Ph. D.)
• Country of Publication: United States
• Language: English

Related Subjects

54 ENVIRONMENTAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
MYCORRHIZAS
SPECIES DIVERSITY
PH VALUE
BIOLOGICAL EFFECTS
CLOVER
NITROGEN
ROOTS
SOIL CHEMISTRY
UPTAKE
VARIATIONS
CHEMISTRY
ELEMENTS
LEGUMINOSAE
NONMETALS
PLANTS
510100* - Environment
Terrestrial- Basic Studies- (-1989)
560302 - Chemicals Metabolism & Toxicology- Microorganisms- (-1987)