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Title: Metabolic imaging with gallium-68- and indium-111-labeled low-density lipoprotein

Low-density lipoprotein (LDL) labeled with either gallium-68 ({sup 68}Ga) or indium-111 ({sup 111}In) was evaluated as a potential PET or SPECT radiopharmaceutical for determination of hepatic lipoprotein metabolism in rabbits. Gallium-68 or {sup 111}In was linked to LDL via diethylenetriaminepentaacetic acid (DTPA) with a 25-70% radiochemical yield. Studies in vivo that compared {sup 68}Ga- or {sup 111}In-DTPA-LDL with dilactitol-({sup 125}I)-tyramine LDL and 131I-LDL showed that both {sup 68}Ga- and {sup 111}In-labeled LDL behaved as residualizing radiotracers. Localization of radioactivity within the liver of normal rabbits was visualized clearly with ({sup 68}Ga)DTPA-LDL by PET and with ({sup 111}In)DTPA-LDL by gamma scintigraphy. Significant differences were observed in hepatic uptake of normal compared with hypercholesterolemic rabbits in which low-capacity LDL receptor-mediated catabolism was saturated. Gallium-68 and {sup 111}In-DTPA-LDL are attractive radiopharmaceuticals for noninvasive delineation of tissue LDL metabolism under normal and pathophysiologic conditions.
Authors:
; ; ;  [1]
  1. (Washington Univ. Medical School, St. Louis, MO (USA))
Publication Date:
OSTI Identifier:
6009188
Resource Type:
Journal Article
Resource Relation:
Journal Name: Journal of Nuclear Medicine; (USA); Journal Volume: 32:2
Country of Publication:
United States
Language:
English
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; LIPOPROTEINS; METABOLISM; RADIOPHARMACEUTICALS; COMPARATIVE EVALUATIONS; DTPA; GALLIUM 68; INDIUM 111; IODINE 125; IODINE 131; LIVER; POSITRON COMPUTED TOMOGRAPHY; RABBITS; SINGLE PHOTON EMISSION COMPUTED TOMOGRAPHY; AMINO ACIDS; ANIMALS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BETA-PLUS DECAY RADIOISOTOPES; BODY; CARBOXYLIC ACIDS; CHELATING AGENTS; COMPUTERIZED TOMOGRAPHY; DAYS LIVING RADIOISOTOPES; DIAGNOSTIC TECHNIQUES; DIGESTIVE SYSTEM; DRUGS; ELECTRON CAPTURE RADIOISOTOPES; EMISSION COMPUTED TOMOGRAPHY; GALLIUM ISOTOPES; GLANDS; HOURS LIVING RADIOISOTOPES; INDIUM ISOTOPES; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOMERIC TRANSITION ISOTOPES; ISOTOPES; LABELLED COMPOUNDS; LIPIDS; MAMMALS; MINUTES LIVING RADIOISOTOPES; NUCLEI; ODD-EVEN NUCLEI; ODD-ODD NUCLEI; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANS; PROTEINS; RADIOISOTOPES; RADIOPROTECTIVE SUBSTANCES; TOMOGRAPHY; VERTEBRATES 550601* -- Medicine-- Unsealed Radionuclides in Diagnostics