The effect of deuterium oxide (D sub 2 O) on in vitro vascular smooth muscle contraction
- Memorial Univ. of Newfoundland, St. John's (Canada)
Deuterium oxide (D{sub 2}O), a stable nonradioactive isotope of water, has been demonstrated to reduce L-type calcium channel conductance in isolated myocytes. Since the concentration of intracellular free calcium has been implicated in the mechanism of vascular smooth muscle contraction, the authors investigated whether it inhibits contraction of vascular smooth muscle. Phenylephrine concentration-contraction curves were carried out in the rat aortic ring preparation to determine whether D{sub 2}O inhibits contraction of rat aorta induced through activation of receptor-operated calcium channels. D{sub 2}O depressed these response curves in a concentration dependent manner with 50% inhibition of maximum contraction observed with 60% D{sub 2}O; this effect proved to be reversible and non-toxic. D{sub 2}O also depressed potassium chloride curves, demonstrating an effect on voltage-operated calcium channels. Since vascular endothelium releases endothelium-derived relaxing factor (EDRF) when stimulated by a range of pharmacological agents, it was examined whether the endothelium has a role in these actions of D{sub 2}O on vascular contraction. Mechanical disruption of the endothelium had no effect.
- OSTI ID:
- 6006722
- Report Number(s):
- CONF-9104107-; CODEN: FAJOE
- Journal Information:
- FASEB Journal (Federation of American Societies for Experimental Biology); (United States), Vol. 4:3; Conference: 75. annual meeting of the Federation of American Societies for Experimental Biology (FASEB), Atlanta, GA (United States), 21-25 Apr 1991; ISSN 0892-6638
- Country of Publication:
- United States
- Language:
- English
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HEAVY WATER
BIOLOGICAL EFFECTS
MUSCLES
CONTRACTION
AORTA
CALCIUM COMPOUNDS
DOSE-RESPONSE RELATIONSHIPS
ENDOTHELIUM
IN VITRO
ION CHANNELING
POTASSIUM CHLORIDES
RATS
RECEPTORS
ALKALI METAL COMPOUNDS
ALKALINE EARTH METAL COMPOUNDS
ANIMAL TISSUES
ANIMALS
ARTERIES
BLOOD VESSELS
BODY
CARDIOVASCULAR SYSTEM
CHANNELING
CHLORIDES
CHLORINE COMPOUNDS
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HALOGEN COMPOUNDS
HYDROGEN COMPOUNDS
MAMMALS
MEMBRANE PROTEINS
ORGANIC COMPOUNDS
ORGANS
OXYGEN COMPOUNDS
POTASSIUM COMPOUNDS
PROTEINS
RODENTS
TISSUES
VERTEBRATES
WATER
560300* - Chemicals Metabolism & Toxicology