Influence of aminooxyacetic acid on the potassium-evoked release of (/sup 3/H)gamma-aminobutyric acid from slices of rat cerebral cortex
The release of (/sup 3/H)GABA from superfused slices of rat cerebral cortex was investigated in the presence and absence of the GABA-transaminase inhibitor aminooxyacetic acid (AOAA). In the latter case, an ion-exchange column chromatographic technique was used to separate (/sup 3/H)GABA from tritiated metabolites released with it into the superfusate. In the absence of AOAA, omission of Ca2+ from the superfusion medium reduced the release of (/sup 3/H)GABA evoked by a 30 mM K+ pulse by 81.6%, whereas in comparable experiments carried out in the presence of AOAA omission of Ca2+ reduced the K+-evoked release by only 23.5%. Similar results were obtained when a 50 mM K+ pulse was used, whereupon omission of Ca2+ reduced (/sup 3/H)GABA release by 78.7% in the absence of AOAA as compared with a reduction of only 47.9% when AOAA was present. It is concluded that the presence of AOAA decreases the Ca2+-dependence of K+-evoked (/sup 3/H)GABA release in this system.
- Research Organization:
- Univ. College, Cardiff, United Kingdom
- OSTI ID:
- 6005443
- Journal Information:
- Neurochem. Res.; (United States), Vol. 9:8
- Country of Publication:
- United States
- Language:
- English
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AMINOBUTYRIC ACID
BIOCHEMICAL REACTION KINETICS
ENZYME INHIBITORS
AMINOTRANSFERASES
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IN VITRO
ION EXCHANGE CHROMATOGRAPHY
METABOLISM
METABOLITES
POTASSIUM COMPOUNDS
RATS
TRACER TECHNIQUES
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AMINO ACIDS
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AUTONOMIC NERVOUS SYSTEM AGENTS
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CHARGED PARTICLES
CHROMATOGRAPHY
DRUGS
ENZYMES
IONS
ISOTOPE APPLICATIONS
KINETICS
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MAMMALS
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NEUROREGULATORS
NITROGEN TRANSFERASES
ORGANIC ACIDS
ORGANIC COMPOUNDS
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550201* - Biochemistry- Tracer Techniques