Injury, nerve, and wound epidermis related electrophoretic and fluorographic protein patterns in forelimbs of adult newts
Polyacrylamide slab gel electrophoresis and (/sup 35/S)methionine fluorography were used to examine proteins in regenerating newt limbs, amputated denervated limbs, unamputated denervated limbs, and separated blastema mesodermal core and wound epidermis. A total of 27 protein electrophoretic bands were obtained from amputated limbs and 24 bands from unamputated limbs. Amputation resulted in the appearance of 4 new bands and the loss of 1 band as compared to unamputated limbs. These 5 banding differences were apparent on stained gels 3 days postamputation and were maintained through 10 weeks postamputation (complete regenerate stage). Only one band in unamputated limbs was always detectable on fluorographs, whereas virtually all of the stainable bands of amputated limbs were visible on fluorographs. Amputation clearly stimulated a marked, generalized increase in the synthesis of limb proteins. The 5 amputation induced changes were equally evident in stained gels of both innervated and denervated limbs. Amputated denervated limbs possessed a full set of fluorographic bands (including the 5 differences) through 18 days postamputation. However, denervation without amputation was not sufficient to alter the stainable banding pattern. Wound epidermis and mesodermal core both displayed the 5 banding differences and had identical banding patterns with the exception of one epidermal specific band. This band was also present in whole limb skin but was absent in unamputated mesodermal limb tissue. This was the only band of unamputated limbs that was consistently detectable by fluorography. It is concluded that amputation induces nerve independent changes in protein synthesis that are common to both mesodermal core and wound epidermis. These changes may represent preparation for cellular proliferation.
- Research Organization:
- Ohio State Univ., Columbus
- OSTI ID:
- 6001263
- Journal Information:
- J. Exp. Zool.; (United States), Vol. 231:2
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
LIMBS
BIOLOGICAL REGENERATION
PROTEINS
BIOSYNTHESIS
ANIMAL TISSUES
ELECTROPHORESIS
EPIDERMIS
FLUOROSCOPY
INJURIES
LABELLED COMPOUNDS
METHIONINE
NERVE CELLS
SALAMANDERS
SKIN
SULFUR 35
TRACER TECHNIQUES
AMINO ACIDS
AMPHIBIANS
ANIMAL CELLS
ANIMALS
AQUATIC ORGANISMS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL RECOVERY
BIOMEDICAL RADIOGRAPHY
BODY
BODY AREAS
CARBOXYLIC ACIDS
DAYS LIVING RADIOISOTOPES
DIAGNOSTIC TECHNIQUES
DRUGS
EPITHELIUM
EVEN-ODD NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
LIPOTROPIC FACTORS
MEDICINE
NUCLEAR MEDICINE
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
RADIOISOTOPES
RADIOLOGY
RECOVERY
SOMATIC CELLS
SULFUR ISOTOPES
SYNTHESIS
TISSUES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques