High-performance liquid chromatography-fluorometric determination of selenium based on selenotrisulfide formation reaction

Journal Article · Wed Feb 01 00:00:00 EST 1989 · Anal. Chem.; (United States)

DOI:https://doi.org/10.1021/ac00178a009· OSTI ID:5996837

Nakagawa, T; Aoyama, E; Hasegawa, N; Kobayashi, N; Tanaka, H

A new high-performance liquid chromatography-fluorometric method has been developed for selective determination of selenium(IV). The method involves precolumn reaction of selenium(IV) with penicillamine (Pen) to produce stable selenotrisulfide (Pen-SSeS-Pen) and subsequent derivatization to a fluorophore by reaction with 7-fluoro-4-nitrobenz-2,1,3-oxadiazole. The fluorophore was separated by reversed-phase HPLC and selenium content was determined by fluorometric detection. The calibration plots showed a linear relationship in the range of 10-2000 ppb of selenium(IV) with a detection limit of 5 ppb (signal to noise ratio (S/N) > 2). The method could determine total content of selenium in biological and environmental samples after digestion of the samples and reduction of selenium(VI) to selenium(IV). The results from standard samples indicated satisfactory agreement with those obtained by other established methods and certified values with good reproducibility. This method is as sensitive as, but simpler in operation than, conventional fluorometry using diaminonaphthalene.

• Research Organization: Kyoto Univ., Kyoto-shi (Japan)
• OSTI ID: 5996837
• Journal Information: Anal. Chem.; (United States), Vol. 61:3
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
SELENIUM
FLUORESCENCE SPECTROSCOPY
LIQUID COLUMN CHROMATOGRAPHY
CALIBRATION
EXPERIMENTAL DATA
INTERFERING ELEMENTS
QUANTITATIVE CHEMICAL ANALYSIS
SELENIUM COMPOUNDS
SULFIDES
TRACE AMOUNTS
CHALCOGENIDES
CHEMICAL ANALYSIS
CHROMATOGRAPHY
DATA
ELEMENTS
EMISSION SPECTROSCOPY
INFORMATION
NUMERICAL DATA
SEMIMETALS
SEPARATION PROCESSES
SPECTROSCOPY
SULFUR COMPOUNDS
400102* - Chemical & Spectral Procedures
400105 - Separation Procedures