Selection and characterization of a glucokinase-deficient mutant of Tetrahymena thermophila

Journal Article · Thu Apr 01 00:00:00 EST 1982 · Mol. Cell. Biol.; (United States)

DOI:https://doi.org/10.1128/MCB.2.4.378· OSTI ID:5996287

Roberts, Jr, C T; Lavine, J E; Morse, D E

The authors isolated a mutant of Tetrahymena thermophila that is resistant to inhibition of growth by the glucose analog 2-deoxyglucose. The mutant exhibits a deficiency in a cytoplasmic glucokinase. This enzymatic defect and the attendant inability to convert 2-deoxyglucose to toxic phosphorylated derivatives is apparently the sole basis for the mutant phenotype since transport of glucose and 2-deoxyglucose is unimpaired; there is no elevation of glucose-6-phosphatase activity, which could decrease the level of toxic 2-deoxyglucose metabolites. Genetic analyses have shown that the mutant allele is recessive and inherited as a single Mendelian mutation. The glucokinase-deficient strain described here is useful for the selection of other mutants in this organism and for the investigation of various cellular processes initiated or modulated by glucose and its analogs. The authors have exploited the molecular defect in this strain to investigate the initial steps in the cyclic AMP-mediated repression of galactokinase gene expression which is caused by glucose.

• Research Organization: Dept. of Biological Sciences and the Marine Science Institute, Univ. of California, Santa Barbara, CA 93106
• OSTI ID: 5996287
• Journal Information: Mol. Cell. Biol.; (United States), Vol. 2:4
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
MUTANTS
PHENOTYPE
PHOSPHOTRANSFERASES
BIOSYNTHESIS
GENE MUTATIONS
TETRAHYMENA
AMP
ANTIMETABOLITES
BIOLOGICAL ADAPTATION
CYTOPLASM
ENZYME INDUCTION
GALACTOSE
GENE REPRESSORS
GENETIC MAPPING
GENETICS
GLUCOSE
GROWTH
INHIBITION
METABOLIC DISEASES
METABOLISM
METABOLITES
MUTATIONS
PHOSPHATASES
SIMULATION
TEMPERATURE EFFECTS
TOXICITY
ALDEHYDES
ANIMALS
BIOLOGY
CARBOHYDRATES
CELL CONSTITUENTS
CILIATA
DISEASES
DRUGS
ENZYMES
ESTERASES
GENE REGULATION
HEXOSES
HYDROLASES
INVERTEBRATES
MAPPING
MICROORGANISMS
MONOSACCHARIDES
NUCLEOPROTEINS
NUCLEOTIDES
ORGANIC COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
PROTEINS
PROTOZOA
SACCHARIDES
SYNTHESIS
TRANSFERASES
550400* - Genetics