Modulation of the estrogen receptor structure, evidence of a heterogeneity

Toulas, C; Guilbaud, N; Delassus, F; Bayard, F; Faye, J C

doi:10.3109/10799899009064654

Title: Modulation of the estrogen receptor structure, evidence of a heterogeneity

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Abstract

In order to analyse the molecular weight polymorphism of the estrogen receptor (ER) in MCF-7 cells, we have developed a procedure which allowed in situ linkage of ER by (3H) tamoxifen aziridine and provided labelled proteins in conditions which minimized protease activities. After labelling, cell lysis was performed in SDS buffer containing various concentrations of mercaptoethanol. Proteins extracted with phenolic solution and precipitated by cold acetone were analysed by SDS PAGE. It appears that beside the form of 67 kDa already described, binding entities of tamoxifen aziridine were also present at a molecular mass of 110 kDa and 45 kDa. On the other hand, investigations on the effect of 12-0-Tetradecanoyl Phorbol 13-Acetate (TPA) showed that TPA induces a decrease of the 67 kDa entity.

Authors:

Toulas, C; Guilbaud, N; Delassus, F; Bayard, F; Faye, J C ^[1]

INSERM U168, CHU Rangueil, Toulouse (France)

Publication Date:: Mon Jan 01 00:00:00 EST 1990

OSTI Identifier:: 5988321

Resource Type:: Journal Article

Journal Name:: Journal of Receptor Research; (USA)

Additional Journal Information:: Journal Volume: 10:1-2; Journal ID: ISSN 0197-5110

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES; 63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; ESTROGENS; RECEPTORS; PHORBOL ESTERS; BIOLOGICAL EFFECTS; MOLECULAR STRUCTURE; ELECTROPHORESIS; ENZYME ACTIVITY; MOLECULAR WEIGHT; PEPTIDE HYDROLASES; TRACER TECHNIQUES; TRITIUM COMPOUNDS; TUMOR CELLS; ANIMAL CELLS; CARCINOGENS; ENZYMES; ESTERS; HORMONES; HYDROGEN COMPOUNDS; HYDROLASES; ISOTOPE APPLICATIONS; MEMBRANE PROTEINS; ORGANIC COMPOUNDS; PROTEINS; STEROID HORMONES; 550201* - Biochemistry- Tracer Techniques; 560300 - Chemicals Metabolism & Toxicology

Citation Formats


                    Toulas, C, Guilbaud, N, Delassus, F, Bayard, F, and Faye, J C. Modulation of the estrogen receptor structure, evidence of a heterogeneity.  United States: N. p., 1990. 
        Web.  doi:10.3109/10799899009064654.

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                    Toulas, C, Guilbaud, N, Delassus, F, Bayard, F, & Faye, J C. Modulation of the estrogen receptor structure, evidence of a heterogeneity.  United States.  https://doi.org/10.3109/10799899009064654

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                    Toulas, C, Guilbaud, N, Delassus, F, Bayard, F, and Faye, J C. 1990.  
        "Modulation of the estrogen receptor structure, evidence of a heterogeneity".  United States.  https://doi.org/10.3109/10799899009064654.

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@article{osti_5988321,

  title        = {Modulation of the estrogen receptor structure, evidence of a heterogeneity},

  author       = {Toulas, C and Guilbaud, N and Delassus, F and Bayard, F and Faye, J C},

  abstractNote = {In order to analyse the molecular weight polymorphism of the estrogen receptor (ER) in MCF-7 cells, we have developed a procedure which allowed in situ linkage of ER by (3H) tamoxifen aziridine and provided labelled proteins in conditions which minimized protease activities. After labelling, cell lysis was performed in SDS buffer containing various concentrations of mercaptoethanol. Proteins extracted with phenolic solution and precipitated by cold acetone were analysed by SDS PAGE. It appears that beside the form of 67 kDa already described, binding entities of tamoxifen aziridine were also present at a molecular mass of 110 kDa and 45 kDa. On the other hand, investigations on the effect of 12-0-Tetradecanoyl Phorbol 13-Acetate (TPA) showed that TPA induces a decrease of the 67 kDa entity.},

  doi          = {10.3109/10799899009064654},

  url          = {https://www.osti.gov/biblio/5988321},
  journal      = {Journal of Receptor Research; (USA)},

  issn         = {0197-5110},

  number       = ,

  volume       = 10:1-2,

  place        = {United States},

  year         = {Mon Jan 01 00:00:00 EST 1990},

  month        = {Mon Jan 01 00:00:00 EST 1990}

}

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