Simultaneous demonstrations of neuropeptide Y gene expression and peptide storage in single neurons of the human brain
Journal Article
·
· Proc. Natl. Acad. Sci. U.S.A.; (United States)
A combination of in situ hybridization for neuropeptide Y mRNA that used a /sup 32/P-labeled complementary RNA probe and immunocytochemistry with polyclonal antibodies against neuropeptide Y were applied to human cortical brain samples to simultaneously localize neuropeptide Y and its mRNA. These two techniques allowed simultaneous identification of neuropeptide Y gene expression and peptide storage in single neutrons of the human brain.
- Research Organization:
- Univ. Hospital, Zuerich (Switzerland)
- OSTI ID:
- 5983518
- Journal Information:
- Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 85:9
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
MESSENGER-RNA
HYBRIDIZATION
NERVE CELLS
CYTOCHEMISTRY
PEPTIDES
GENE REGULATION
BRAIN
PHOSPHORUS 32
TRANSCRIPTION
ANIMAL CELLS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMISTRY
BODY
CENTRAL NERVOUS SYSTEM
CHEMISTRY
DAYS LIVING RADIOISOTOPES
ISOTOPES
LIGHT NUCLEI
NERVOUS SYSTEM
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
PHOSPHORUS ISOTOPES
PROTEINS
RADIOISOTOPES
RNA
SOMATIC CELLS
550201* - Biochemistry- Tracer Techniques
MESSENGER-RNA
HYBRIDIZATION
NERVE CELLS
CYTOCHEMISTRY
PEPTIDES
GENE REGULATION
BRAIN
PHOSPHORUS 32
TRANSCRIPTION
ANIMAL CELLS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMISTRY
BODY
CENTRAL NERVOUS SYSTEM
CHEMISTRY
DAYS LIVING RADIOISOTOPES
ISOTOPES
LIGHT NUCLEI
NERVOUS SYSTEM
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
PHOSPHORUS ISOTOPES
PROTEINS
RADIOISOTOPES
RNA
SOMATIC CELLS
550201* - Biochemistry- Tracer Techniques