Use of synthetic oligonucleotides for genomic DNA dot hybridization to split the DQw3 haplotype

Journal Article · Fri Apr 01 00:00:00 EST 1988 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.85.8.2682· OSTI ID:5983440

Martell, M; Le Gall, I; Millasseau, P; Dausset, J; Cohen, D

Comparison of two different HLA-DQ..beta..gene sequences from two DR4 individuals, probably corresponding to DQw3.2 (DQR4) and DQw3.1 (DQR5) specificities, has shown several nucleotide variations. Eight oligonucleotides (24 bases long), derived from these polymorphic areas, have been synthesized. Each oligonucleotide was hybridized to BamHI-digested DNA samples from eight families with HLA-DR4 individuals. Four polymorphic BamHI fragments were detected. Two of eight oligonucleotides gave a single signal (8.9 kilobases) on DQw3.2-positive haplotypes. The authors used one of these oligonucleotides in a genomic DNA dot hybridization and detected a hybridization signal only in DQw3.2-positive individuals. A very simple test like this allows the screening of a large population sample within a very short period.

• Research Organization: Centre d'Etude du Polymorphisme Humain, Paris (France)
• OSTI ID: 5983440
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 85:8
• Country of Publication: United States
• Language: English

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