Semipermissive replication of a nuclear polyhedrosis virus of Autographa californica in a gypsy moth cell line
Several gypsy moth cell lines have been previously described as nonpermissive for the multiple-embedded nuclear polyhedrosis virus of Autographa californica (AcMNPV). In this report, the authors demonstrate the semipermissive infection of a gypsy moth cell line, IPLB-LD-652Y, with AcMNPV. IPLB-LD-652Y cells infected with AcMNPV produced classic cytopathic effects but failed to yield infectious progeny virus. Results of experiments employing DNA-DNA dot hybridization suggested that AcMNPV DNA synthesis was initiated from 8 to 12 h postinfection (p.i.), continued at a maximum rate from 12 to 20 h p.i., and declined from 20 to 36 h p.i. The rate of AcMNPV DNA synthesis approximated that observed in the permissive TN-368 cell line. AcMNPV-infected IPLB-LD-652Y cells, pulse-labeled with (/sup 35/S)methionine at various time intervals p.i. and analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, revealed four virus-induced proteins, one novel to the semipermissive system and three early ..cap alpha.. proteins, synthesized from 1 to 20 h p.i. Thereafter, both host and viral protein synthesis was completely suppressed. These results suggest that AcMNPV adsorbed, penetrated, and initiated limited macromolecular synthesis in the semipermissive gypsy moth cell line. However, the infection cycle was restricted during the early phase of AcMNPV replication.
- Research Organization:
- Agricultural Research Service, Beltsville, MD (USA)
- OSTI ID:
- 5982648
- Journal Information:
- J. Virol.; (United States), Vol. 57:1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
DNA REPLICATION
BIOCHEMISTRY
VIRUSES
HYBRIDIZATION
CELL CULTURES
ELECTROPHORESIS
METHIONINE
MOTHS
NUCLEOTIDES
PHOSPHORUS 32
PLAQUE FORMATION
PROTEINS
SULFUR 35
AMINO ACIDS
ANIMALS
ARTHROPODS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOXYLIC ACIDS
CHEMISTRY
DAYS LIVING RADIOISOTOPES
DRUGS
EVEN-ODD NUCLEI
INSECTS
INVERTEBRATES
ISOTOPES
LEPIDOPTERA
LIGHT NUCLEI
LIPOTROPIC FACTORS
MICROORGANISMS
NUCLEI
NUCLEIC ACID REPLICATION
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PARASITES
PHOSPHORUS ISOTOPES
RADIOISOTOPES
SULFUR ISOTOPES
550201* - Biochemistry- Tracer Techniques