Bioprocessing of sweet sorghum with in situ-produced enzymes
- Colorado State Univ., Fort Collins, CO (United States)
- Technical Univ. of Budapest (Hungary)
- Pannon Agrarian Univ., Mosonmagyarovar (Hungary)
Enzyme-assisted ensiling (ENLAC), using in situ-produced enzymes from Gliocladium sp. TUB-F-498, preserved 80% of the sugar content of sweet sorghum, and facilitated its extraction by countercurrent diffusion. The in situ enzyme was produced on the extracted sweet sorghum pulp by an 8-d solid substrate fermentation (SSF) with a yield of 4.6 cellulose and 400 IU/g dry wt xylanase. Two percent of the fermented substrate had cellulose and xylanase levels equivalent or superior to levels found in the commercial enzymes Celluclast and Viscozyme Novo at the 0.025% application level in ENLAC. The in situ-production of enzymes on recyclable substrates may reduce bioprocessing costs significantly. In this ENLAC process, the cost of the in situ enzymes is estimated to be about $0.12/metric ton (MT) substrate, compared to $9.5/metric ton for the commercial enzymes, a cost reduction of nearly 80-fold. 4 refs., 3 figs., 4 tabs.
- Sponsoring Organization:
- USDOE
- OSTI ID:
- 598225
- Report Number(s):
- CONF-950587-; ISSN 0273-2289; CNN: Grant LG 920571; TRN: 98:001503-0014
- Journal Information:
- Applied Biochemistry and Biotechnology, Vol. 57-58; Conference: 17. symposium on biotechnology for fuels and chemicals, Vail, CO (United States), 7-11 May 1995; Other Information: PBD: Spr 1996
- Country of Publication:
- United States
- Language:
- English
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