Partial characterization of GTP-binding proteins in Neurospora

Journal Article · Fri Aug 14 00:00:00 EDT 1987 · Biochem. Biophys. Res. Commun.; (United States)

DOI:https://doi.org/10.1016/0006-291X(87)90772-8· OSTI ID:5977976

Hasunuma, K; Miyamoto-Shinohara, Y; Furukawa, K

Six fractions of GTP-binding proteins separated by gel filtration of a mycelial extract containing membrane components of Neurospora crassa were partially characterized. (/sup 35/S)GTP gamma S bound to GTP-binding protein was assayed by repeated treatments with a Norit solution and centrifugation. The binding of (/sup 35/S)GTP gamma S to GTP-binding proteins was competitively prevented in the presence of 0.1 to 1 mM GTP but not in the presence of ATP. These GTP-binding proteins fractionated by the gel column had Km values of 20, 7, 4, 4, 80 and 2 nM. All six fractions of these GTP-binding proteins showed the capacity to be ADP-ribosylated by pertussis toxin.

• Research Organization: National Institute for Basic Biology, Okazaki, Japan
• OSTI ID: 5977976
• Journal Information: Biochem. Biophys. Res. Commun.; (United States), Vol. 146:3
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
PROTEINS
BIOCHEMICAL REACTION KINETICS
CHEMICAL COMPOSITION
ATP
CELL MEMBRANES
CENTRIFUGATION
FRACTIONATION
NEUROSPORA
NUCLEOTIDES
SULFUR 35
TOXINS
TRACER TECHNIQUES
ANTIGENS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CELL CONSTITUENTS
DAYS LIVING RADIOISOTOPES
EVEN-ODD NUCLEI
FUNGI
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LIGHT NUCLEI
MATERIALS
MEMBRANES
NUCLEI
ORGANIC COMPOUNDS
PLANTS
RADIOISOTOPES
REACTION KINETICS
SEPARATION PROCESSES
SULFUR ISOTOPES
TOXIC MATERIALS
550201* - Biochemistry- Tracer Techniques