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Title: Effect of storage media on the proliferation of periodontal ligament fibroblasts

Abstract

The effect of storage media, which are routinely used in replantation, upon the proliferative capacity of periodontal ligament fibroblasts, was compared with the effect of a tissue culture medium. The periodontal tissue was obtained from mandibular central incisors of White New Zealand rabbits. The experiments were performed in fibroblasts derived during second subculture. The storage media were physiologic salt solution, Ringer's solution and Rivanol; the tissue culture medium was alpha-minimum essential medium without nucleosides. The incubation period was 1 hour. (/sup 3/H)-thymidine incorporation and cell counts were taken to indicate changes in the proliferative capacity of the fibroblasts. The tissue culture experiments showed that the proliferative ability of the periodontal ligament fibroblasts was dependent upon the composition of the storage medium. Physiologic salt solution, Ringer's solution and Rivanol were unable to maintain the metabolism of the fibroblasts. alpha-MEM medium, however, was capable of stimulating proliferation of the periodontal ligament fibroblasts.

Authors:
; ; ;
Publication Date:
Research Org.:
Poliklinik fuer Zahnaerztliche Prothetik, Muenchen, Germany, F.R.
OSTI Identifier:
5977732
Resource Type:
Journal Article
Journal Name:
J. Periodontol.; (United States)
Additional Journal Information:
Journal Volume: 58:7
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; CULTURE MEDIA; CHEMICAL COMPOSITION; FIBROBLASTS; CELL PROLIFERATION; LIGAMENTS; IMPLANTS; RABBITS; STORAGE; TEETH; THYMIDINE; TISSUE CULTURES; TRACER TECHNIQUES; TRITIUM COMPOUNDS; ANIMAL CELLS; ANIMAL TISSUES; ANIMALS; AZINES; BODY; CONNECTIVE TISSUE; CONNECTIVE TISSUE CELLS; DIGESTIVE SYSTEM; HETEROCYCLIC COMPOUNDS; ISOTOPE APPLICATIONS; LABELLED COMPOUNDS; MAMMALS; NUCLEOSIDES; NUCLEOTIDES; ORAL CAVITY; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; PYRIMIDINES; RIBOSIDES; SOMATIC CELLS; TISSUES; VERTEBRATES; 550301* - Cytology- Tracer Techniques

Citation Formats

Lauer, H C, Mueller, J G, Gross, J, and Horster, M F. Effect of storage media on the proliferation of periodontal ligament fibroblasts. United States: N. p., 1987. Web. doi:10.1902/jop.1987.58.7.481.
Lauer, H C, Mueller, J G, Gross, J, & Horster, M F. Effect of storage media on the proliferation of periodontal ligament fibroblasts. United States. https://doi.org/10.1902/jop.1987.58.7.481
Lauer, H C, Mueller, J G, Gross, J, and Horster, M F. 1987. "Effect of storage media on the proliferation of periodontal ligament fibroblasts". United States. https://doi.org/10.1902/jop.1987.58.7.481.
@article{osti_5977732,
title = {Effect of storage media on the proliferation of periodontal ligament fibroblasts},
author = {Lauer, H C and Mueller, J G and Gross, J and Horster, M F},
abstractNote = {The effect of storage media, which are routinely used in replantation, upon the proliferative capacity of periodontal ligament fibroblasts, was compared with the effect of a tissue culture medium. The periodontal tissue was obtained from mandibular central incisors of White New Zealand rabbits. The experiments were performed in fibroblasts derived during second subculture. The storage media were physiologic salt solution, Ringer's solution and Rivanol; the tissue culture medium was alpha-minimum essential medium without nucleosides. The incubation period was 1 hour. (/sup 3/H)-thymidine incorporation and cell counts were taken to indicate changes in the proliferative capacity of the fibroblasts. The tissue culture experiments showed that the proliferative ability of the periodontal ligament fibroblasts was dependent upon the composition of the storage medium. Physiologic salt solution, Ringer's solution and Rivanol were unable to maintain the metabolism of the fibroblasts. alpha-MEM medium, however, was capable of stimulating proliferation of the periodontal ligament fibroblasts.},
doi = {10.1902/jop.1987.58.7.481},
url = {https://www.osti.gov/biblio/5977732}, journal = {J. Periodontol.; (United States)},
number = ,
volume = 58:7,
place = {United States},
year = {Wed Jul 01 00:00:00 EDT 1987},
month = {Wed Jul 01 00:00:00 EDT 1987}
}