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Title: Bivariate flow cytometric analysis of murine intestinal epithelial cells for cytokinetic studies

The heterogeneous nature of the small intestine and the lack of methods to obtain pure crypt populations has, in the past, limited the application of standard flow cytometric analysis for cytokinetic studies of the proliferating crypts. The authors describe a flow cytometric technique to discriminate crypt and villus cells in an epithelial cell suspension on the basis of cell length, and to measure the DNA content of the discriminated subpopulations. These data indicate that bivariate analysis of a mixed epithelial cell suspension can be used to distinguish mature villus cells, G/sub 1/ crypt cells, and S-phase crypt cells. In addition, continuous labeling studies suggest that the position of a cell on the cell length axis reflects epithelial cell maturity. The authors applied this flow cytometric technique to determine the cytokinetic nature of epithelial cells obtained by sequential digestion of the small intestine. 22 references, 4 figures, 2 tables.
Authors:
; ;
Publication Date:
OSTI Identifier:
5965803
DOE Contract Number:
W-7405-ENG-48
Resource Type:
Journal Article
Resource Relation:
Journal Name: Cytometry (Baltimore); (United States); Journal Volume: 5
Research Org:
Lawrence Livermore National Lab., CA
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; EPITHELIUM; CELL PROLIFERATION; CELL FLOW SYSTEMS; DNA; EXPERIMENTAL DATA; MICE; MULTIVARIATE ANALYSIS; SMALL INTESTINE; ANIMAL TISSUES; ANIMALS; BODY; DATA; DIGESTIVE SYSTEM; GASTROINTESTINAL TRACT; INFORMATION; INTESTINES; MAMMALS; MATHEMATICS; NUCLEIC ACIDS; NUMERICAL DATA; ORGANIC COMPOUNDS; ORGANS; RODENTS; STATISTICS; TISSUES; VERTEBRATES 550300* -- Cytology