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Title: Increased 5. cap alpha. -reductase activity in idiopathic hirsutism

In vitro, genital skin 5..cap alpha..-reductase activity (5..cap alpha..-RA) was measured in ten hirsute women with normal androgen levels (idiopathic hirsutism (IH)) and in ten hirsute women with elevated androgen levels (polycystic ovary syndrome (PCO)) in order to determine the influence of secreted androgens on 5..cap alpha..-RA. In vitro 5..cap alpha..-RA was assessed by incubations of skin with /sup 14/C-testosterone (T) for 2 hours, after which steroids were separated and the radioactivity of dihydrotestosterone (DHT) and 5..cap alpha..-androstane 3..cap alpha..-17..beta..-estradiol (3..cap alpha..-diol) in specific eluates were determined. All androgens were normal in IH with the exception of higher levels of 3..cap alpha..-diol glucuronide which were similar to the levels of PCO. The conversion ratio (CR) of T to DHT in IH and PCO were similar, yet significantly greater than the CR of control subjects. The CR of T to 3..cap alpha..-diol in IH and PCO were similar, yet higher than in control subjects. Serum androgens showed no correlation with 5..cap alpha..-RA, while the CR of T to DHT showed a significant positive correlation with the Ferriman and Gallwey score. The increased 5..cap alpha..-RA in IH appears to be independent of serum androgen levels and is, therefore, an inherent abnormality. Themore » term idiopathic is a misnomer, because hirsutism in these patients may be explained on the basis of increased skin 5..cap alpha..-RA.« less
Authors:
;
Publication Date:
OSTI Identifier:
5964611
Resource Type:
Journal Article
Resource Relation:
Journal Name: Fert. Steril.; (United States); Journal Volume: 43:1
Research Org:
Univ. of Southern California School of Medicine, Los Angeles
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; OXIDOREDUCTASES; ENZYME ACTIVITY; SKIN; SAMPLING; SKIN DISEASES; ETIOLOGY; ANDROGENS; ANDROSTANES; CARBON 14 COMPOUNDS; ENDOCRINE DISEASES; ESTRADIOL; FEMALE GENITALS; HAIR; IN VITRO; PATIENTS; TESTOSTERONE; TRACER TECHNIQUES; WOMEN; ANIMALS; BODY; DISEASES; ENZYMES; ESTRANES; ESTROGENS; FEMALES; HORMONES; HYDROXY COMPOUNDS; ISOTOPE APPLICATIONS; KETONES; LABELLED COMPOUNDS; MAMMALS; MAN; ORGANIC COMPOUNDS; ORGANS; PRIMATES; STEROID HORMONES; STEROIDS; VERTEBRATES 551001* -- Physiological Systems-- Tracer Techniques