Comparison between NO sub x evolution mechanisms of wild-type and nr sub 1 mutant soybean leaves. [Glycine max (L. ) Merr]

Journal Article · Tue May 01 00:00:00 EDT 1990 · Plant Physiology; (USA)

DOI:https://doi.org/10.1104/pp.93.1.26· OSTI ID:5963707

Klepper, L ^[1]

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1. Univ. of Nebraska, Lincoln (USA)

The nr{sub 1} soybean (Glycine max (L.) Merr.) mutant does not contain the two constitutive nitrate reductases, one of which is responsible for enzymic conversion of nitrite to NO{sub x} (NO + NO{sub 2}). It was tested for possible nonenzymic NO{sub x} formation and evolution because of known chemical reactions between NO{sub 2}{sup {minus}} and plant metabolites and the instability of nitrous acid. It did not evolve NO{sub x} during the in vivo NR assay, but intact leaves did evolve small amounts of NO{sub x} under dark, anaerobic conditions. Experiments were conducted to compare NO{sub 3}{sup {minus}} reduction, NO{sub 2}{sup {minus}} accumulation, and the NO{sub x} evolution processes of the wild type (cv Williams) and the nr{sub 1} mutant. This report concludes that NO{sub x} evolution by the nr{sub 1} mutant was due to nonenzymic, chemical reactions between plant metabolites and accumulated NO{sub 2}{sup {minus}} and/or decomposition of nitrous acid. Nonenzymic NO{sub x} evolution probably also occurs in wild type to a degree but could be easily masked by high rates of the enzymic process.

• OSTI ID: 5963707
• Journal Information: Plant Physiology; (USA), Vol. 93:1; ISSN 0032-0889
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GLYCINE HISPIDA
PHYSIOLOGY
NITROGEN OXIDES
BIOCHEMICAL REACTION KINETICS
BIODEGRADATION
CHEMICAL REACTIONS
COMPARATIVE EVALUATIONS
LEAVES
METABOLITES
MUTANTS
NITROGEN DIOXIDE
NITROUS OXIDE
CHALCOGENIDES
DECOMPOSITION
KINETICS
LEGUMINOSAE
MAGNOLIOPHYTA
MAGNOLIOPSIDA
NITROGEN COMPOUNDS
OXIDES
OXYGEN COMPOUNDS
PLANTS
REACTION KINETICS
550200* - Biochemistry