Use of /sup 14/CO/sub 2/ ratios in metabolic assessment of human spermatozoa

Conference · Fri May 01 00:00:00 EDT 1987 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5963602

Holleran, A L; Mendez, C M; Kelleher, J K; Naz, R K

Comparison of /sup 14/CO/sub 2/ production for (1-/sup 14/C), (2-/sup 14/C) and (3-/sup 14/C) pyruvate indicates the metabolic fate of pyruvate. Assuming that all pyruvate oxidized enters the TCA cycle via pyruvate dehydrogenase, the ratio of steady state /sup 14/CO/sub 2/ production, (2-/sup 14/C) pyruvate: (3-/sup 14/C) pyruvate, determines the probability that specific citrate carbons will complete a turn of the TCA cycle. Comparing this probability and the /sup 14/CO/sub 2/ production from (1-/sup 14/C) pyruvate estimates the flux pyruvate to products derived from acetate that do not enter the TCA cycle. Data was collected for human sperm metabolizing glutamine and pyruvate over a four-hour period. The ratio of /sup 14/CO/sub 2/ production, (2-/sup 14/C) pyruvate: (3-/sup 14/C) pyruvate even when correction was made for the fact that not all carbon derived from (2-/sup 14/C) pyruvate that enters the TCA cycle is converted to CO/sub 2/. /sup 14/CO/sub 2/ production from (U-/sup 14/C) glutamine was linear for glutamine concentration below 0.5 mM. In conclusion, CO/sub 2/ ratios methods are applicable in metabolic analysis of small samples of human sperm where metabolite measurements are impractical.

• Research Organization: George Washington Univ. Medical Center, Washington, DC
• OSTI ID: 5963602
• Report Number(s): CONF-870644-; TRN: 87-039131
• Journal Information: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 46:6; Conference: 78. annual meeting of the American Society of Biological Chemists conference, Philadelphia, PA, USA, 7 Jun 1987
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GLUTAMINE
BIOCHEMICAL REACTION KINETICS
PYRUVIC ACID
SPERMATOZOA
METABOLISM
CARBON 14 COMPOUNDS
CARBON DIOXIDE
KREBS CYCLE
OXIDATION
OXIDOREDUCTASES
TRACER TECHNIQUES
AMIDES
AMINO ACIDS
CARBON COMPOUNDS
CARBON OXIDES
CARBOXYLIC ACIDS
CHALCOGENIDES
CHEMICAL REACTIONS
ENZYMES
GAMETES
GERM CELLS
ISOTOPE APPLICATIONS
KETO ACIDS
KINETICS
LABELLED COMPOUNDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXIDES
OXYGEN COMPOUNDS
REACTION KINETICS
550501* - Metabolism- Tracer Techniques