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Title: Transforming growth factor (TGF)-. alpha. in human milk

Transforming growth factor (TGF)-{alpha} and epidermal growth factor (EGF) were measured in human milk by means of homologous radioimmunoassay. As previously reported, EGF concentration in the colostrum was approximately 200 ng/ml and decreased to 50 ng/ml by day 7 postpartum. The value of immunoreactive (IR)-TGF-{alpha} was 2.2-7.2 ng/ml, much lower than that of EGF. In contrast to EGF, the concentration of IR-TGF-{alpha} was fairly stable during the 7 postpartum days. There was no relationship between the concentrations of IR-TGF-{alpha} and IR-EGF, suggesting that the regulatory mechanism in the release of the two growth factors is different. On gel-chromatography using a Sephadex G-50 column, IR-EGF appeared in the fraction corresponding to that of authentic human EGF, while 70%-80% of the IR-TGF-{alpha} was eluted as a species with a molecular weight greater than that of authentic human TGF-{alpha}. Although the physiological role of TGF-{alpha} in milk is not known, it is possible that it is involved in the development of the mammary gland and/or the growth of newborn infants.
Authors:
; ;  [1] ;  [2] ; ; ; ; ;
  1. (Research Institute for Growth Sciences, Tokyo (Japan))
  2. (Tokyo Women's Medical College (Japan))
Publication Date:
OSTI Identifier:
5962990
Resource Type:
Journal Article
Resource Relation:
Journal Name: Life Sciences; (USA); Journal Volume: 48:12
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; GROWTH FACTORS; RADIOIMMUNOASSAY; BIOLOGICAL FUNCTIONS; BIOSYNTHESIS; GEL PERMEATION CHROMATOGRAPHY; MAN; MILK; MOLECULAR WEIGHT; ANIMALS; BIOASSAY; BIOLOGICAL MATERIALS; BODY FLUIDS; CHROMATOGRAPHY; DIAGNOSTIC TECHNIQUES; FOOD; FUNCTIONS; IMMUNOASSAY; IMMUNOLOGY; ISOTOPE APPLICATIONS; MAMMALS; MATERIALS; MITOGENS; ORGANIC COMPOUNDS; PRIMATES; PROTEINS; RADIOASSAY; RADIOIMMUNODETECTION; RADIOIMMUNOLOGY; SEPARATION PROCESSES; SYNTHESIS; TRACER TECHNIQUES; VERTEBRATES 550201* -- Biochemistry-- Tracer Techniques