65zinc uptake from blood into brain and other tissues in the rat

Journal Article · Mon Oct 01 00:00:00 EDT 1990 · Neurochemical Research; (USA)

DOI:https://doi.org/10.1007/BF00965746· OSTI ID:5950557

Pullen, R G; Franklin, P A; Hall, G H ^[1]

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1. Sunderland Polytechnic, Tyne Wear (England)

Zinc is essential for normal growth, development and brain function although little is known about brain zinc homeostasis. Therefore, in this investigation we have studied 65Zn uptake from blood into brain and other tissues and have measured the blood-brain barrier permeability to 65Zn in the anaesthetized rat in vivo. Adult male Wistar rats within the weight range 500-600 g were used. 65ZnCl2 and (125I)albumin, the latter serving as a vascular marker, were injected in a bolus of normal saline I.V. Sequential arterial blood samples were taken during experiments that lasted between 5 min and 5 hr. At termination, samples from the liver, spleen, pancreas, lung, heart, muscle, kidney, bone, testis, ileum, blood cells, csf, and whole brain were taken and analysed for radio-isotope activity. Data have been analysed by Graphical Analysis which suggests 65Zn uptake from blood by all tissues sampled was unidirectional during this experimental period except brain, where at circulation times less than 30 min, 65Zn fluxes were bidirectional. In addition to the blood space, the brain appears to contain a rapidly exchanging compartment(s) for 65Zn of about 4 ml/100g which is not csf.

• OSTI ID: 5950557
• Journal Information: Neurochemical Research; (USA), Vol. 15:10; ISSN 0364-3190
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
BLOOD-BRAIN BARRIER
PERMEABILITY
ZINC
METABOLISM
ALBUMINS
BIOLOGICAL MARKERS
HOMEOSTASIS
IODINE 125
ISOTOPE DILUTION
RATS
REGRESSION ANALYSIS
TISSUE DISTRIBUTION
ZINC 65
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-PLUS DECAY RADIOISOTOPES
DAYS LIVING RADIOISOTOPES
DISTRIBUTION
ELECTRON CAPTURE RADIOISOTOPES
ELEMENTS
EVEN-ODD NUCLEI
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MATHEMATICS
METALS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PROTEINS
RADIOISOTOPES
RODENTS
STATISTICS
TRACER TECHNIQUES
VERTEBRATES
ZINC ISOTOPES
560300* - Chemicals Metabolism & Toxicology
550501 - Metabolism- Tracer Techniques